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Magnetic nanoparticles for the affinity adsorption of maltose binding protein (MBP) fusion enzymes

, 2012
By the combination of fusion protein technology and nanoparticle surface modification technology, a maltose-modified magnetic support has been successfully developed for the integration of separation/purification, and immobilization of maltose binding ...
Lilin Zhou   +7 more
semanticscholar   +1 more source

Tritium NMR spectroscopy of ligand binding to maltose-binding protein

Biochemistry, 1991
Tritium-labeled alpha- and beta-maltodextrins have been used to study their complexes with maltose-binding protein (MBP), a 40-kDa bacterial protein. Five substrates, from maltose to maltohexaose, were labeled at their reducing ends and their binding studied.
Philip G. Williams   +4 more
openaire   +3 more sources

Associative properties of the Escherichiacoli galactose binding protein and maltose binding protein

Biochemical and Biophysical Research Communications, 1982
The ligand-binding characteristics of periplasmic galactose-binding protein and maltose-binding protein of Escherichia coli are analyzed. The saturation function was decreased upon increasing protein concentration and the monomer-dimer equilibrium was shifted towards the monomeric protein form upon an increase of the ligand concentration.
openaire   +5 more sources

Molecular Imprinting of Maltose Binding Protein: Tuning Protein Recognition at the Molecular Level

, 2011
Protein imprinting in hydrogels is one approach for developing artificial receptors capable of specific recognition and binding of a target molecule. Through selection of monomers with side groups that can interact with the target protein and control ...
M. Zayats   +3 more
semanticscholar   +1 more source

Purification of Proteins Fused to Maltose-Binding Protein.

Methods in molecular biology, 2017
M. Lebendiker, T. Danieli
semanticscholar   +1 more source

An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein.

Gene, 1988
C. V. Maina   +7 more
semanticscholar   +1 more source

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