Results 21 to 30 of about 461 (119)
Pioneering role of RNA in the early evolution of life. [PDF]
Genet Mol BiolThe catalytic, regulatory and structural properties of RNA, combined with their extraordinary ubiquity in cellular processes, are consistent with the proposal that this molecule played a much more conspicuous role in heredity and metabolism during the ...
Muñoz-Velasco I +6 more
europepmc +4 more sources
Improving the Ribozyme Toolbox: From Structure-Function Insights to Synthetic Biology Applications [PDF]
, 2022 Self-cleaving ribozymes are a naturally occurring class of catalytically active RNA molecules which cleave their own phosphate backbone. In nature, self-cleaving ribozymes are best known for their role in processing concatamers of viral genomes into ...
Roberts, Jessica Michelle
core +1 more source
Intronic hammerhead ribozymes in mRNA biogenesis [PDF]
, 2012 [EN] Small self-cleaving ribozymes are a group of natural RNAs that are capable of catalyzing their own and sequence-specific endonucleolytic cleavage.
García Robles, Inmaculada +2 more
core +1 more source
Controlling Gene Expression in Mammalian Cells Using Multiplexed Conditional Guide RNAs for Cas12a
bioRxiv, 2021 Spatiotemporal control of the activity of Cas proteins is of considerable interest for both basic research and therapeutics. Only few mechanisms have been demonstrated for regulating the activity of guide RNAs (gRNAs) for Cas12a in mammalian cells ...
Lukas Oesinghaus, F. Simmel
semanticscholar +1 more source
Identification of an hepatitis delta virus-like ribozyme at the mRNA 5′-end of the L1Tc retrotransposon from Trypanosoma cruzi [PDF]
, 2011 L1Tc is a non-LTR LINE element from Trypanosoma cruzi that encodes its transposition machinery and bears an internal promoter. Herewith, we report the identification of an in vitro active hepatitis delta virus-like ribozyme located in the first 77 nt at ...
Alonso, Carlos +4 more
core +2 more sources
Design of Mammalian ON-Riboswitches Based on Tandemly Fused Aptamer and Ribozyme.
ACS Synthetic Biology, 2019 Self-cleaving ribozymes engineered to be activated or inhibited by a small molecule binding to an RNA aptamer inserted within a ribozyme (aptazymes) have proven to be useful for controlling gene expression in living cells. In mammalian cells, an aptazyme
Kamila Mustafina +2 more
semanticscholar +1 more source
CPEB3 regulates neuron-specific alternative splicing and involves neurogenesis gene expression
Aging, 2020 In the mammalian brain, alternative pre-mRNA splicing is a fundamental mechanism that modifies neuronal function dynamically where secretion of different splice variants regulates neurogenesis, development, pathfinding, maintenance, migration, and ...
W. Qu +6 more
semanticscholar +1 more source
Evaluating Performance of Different RNA Secondary Structure Prediction Programs Using Self-cleaving Ribozymes [PDF]
Accurate identification of the correct, biologically relevant RNA structures is critical to understanding various aspects of RNA biology since proper folding represents the key to the functionality of all types of RNA molecules and plays pivotal roles in
Chen, Junjie +6 more
core +2 more sources
ACS Synthetic Biology, 2018 Self-cleaving ribozymes, in combination with aptamers and various classes of RNAs, have been heavily engineered to create RNA devices to control gene expression.
Shungo Kobori, Y. Yokobayashi
semanticscholar +1 more source