Results 51 to 60 of about 461 (119)

Significantly higher activity of a cytoplasmic hammerhead ribozyme than a corresponding nuclear counterpart: engineered tRNAs with an extended 3' end can be exported efficiently and specifically to the cytoplasm in mammalian cells

Nucleic Acids Res., 2001
Hammerhead ribozymes were expressed under the control of similar tRNA promoters, localizing transcripts either in the cytoplasm or the nucleus. The tRNA(Val)-driven ribozyme (tRNA-Rz; tRNA with extra sequences at the 3' end) that has been used in our ...
T. Kuwabara, M. Warashina, S. Koseki, M. Sano, J. Ohkawa, K. Nakayama, K. Taira   +6 more
semanticscholar   +1 more source

Convergent Evolution of Adenosine Aptamers Spanning Bacterial, Human, and Random Sequences Revealed by Structure-Based Bioinformatics and Genomic SELEX [PDF]

, 2012
SummaryAptamers are structured macromolecules in vitro evolved to bind molecular targets, whereas in nature they form the ligand-binding domains of riboswitches.
Jameson, Nora E., Larralde-Ridaura, Rosa, Lin, Dana, Lupták, Andrej, Masuda, Stuart J., Vu, Michael M.K.   +5 more
core   +1 more source

A Tetrahymena thermophila ribozyme-based indicator gene to detect transposition of marked retroelements in mammalian cells.

Nucleic Acids Research, 2002
We devised an indicator gene for retrotransposition based on an autocatalytic ribozyme element--the Tetrahymena thermophila 23S rRNA group I intron--which can self-splice in vitro and does not require--at variance with nuclear mRNA introns--any specific ...
Cécile Esnault, J. Casella, T. Heidmann   +2 more
semanticscholar   +1 more source

Identification of inhibitors of ribozyme self-cleavage in mammalian cells via high-throughput screening of chemical libraries.

RNA: A publication of the RNA Society, 2006
We have recently described an RNA-only gene regulation system for mammalian cells in which inhibition of self-cleavage of an mRNA carrying ribozyme sequences provides the basis for control of gene expression.
Laising Yen, M. Magnier, R. Weissleder, B. Stockwell, R. Mulligan   +4 more
semanticscholar   +1 more source

The Metabolome Weakens RNA Thermodynamic Stability and Strengthens RNA Chemical Stability. [PDF]

Biochemistry, 2022
Sieg JP, McKinley LN, Huot MJ, Yennawar NH, Bevilacqua PC.   +4 more
europepmc   +1 more source

Predicting higher-order mutational effects in an RNA enzyme by machine learning of high-throughput experimental data. [PDF]

Front Mol Biosci, 2022
Beck JD, Roberts JM, Kitzhaber JM, Trapp A, Serra E, Spezzano F, Hayden EJ.   +6 more
europepmc   +1 more source

Relationships between the Activities in Vitro and in Vivo of Various Kinds of Ribozyme and Their Intracellular Localization in Mammalian Cells*

Journal of Biological Chemistry, 2001
Nineteen different functional RNAs were synthesized for an investigation of the actions of ribozymes, in vitro and in vivo, under the control of two different promoters, tRNA or U6, which localize transcripts either in the cytoplasm or in the nucleus. No
Y. Kato, T. Kuwabara, M. Warashina, Hirofumi Toda, K. Taira   +4 more
semanticscholar   +1 more source

Generation of a Mammalian Cell Line Deficient in Glucose-regulated Protein Stress Induction through Targeted Ribozyme Driven by a Stress-inducible Promoter (*)

Journal of Biological Chemistry, 1995
GRP94 is an endoplasmic reticulum (ER) localized glycoprotein with Cabinding and protein chaperoning properties. Using a ribozyme driven by a stress-inducible promoter and targeted against grp94 mRNA, we have generated a cell line deficient in its ...
E. Little, Amy S. Lee
semanticscholar   +1 more source

Automated Design and Empirical Validation of Hammerhead Ribozymes [PDF]

, 2015
Ribozymes are catalytic RNA molecules. Hammerhead ribozymes are one of a set of ribozymes capable of cleaving RNA molecules, in cis and in trans, without the help of other molecules, such as proteins.
Kharma, Nawwaf
core  

Mining metatranscriptomes reveals a vast world of viroid-like circular RNAs. [PDF]

Cell, 2023
Lee BD, Neri U, Roux S, Wolf YI, Camargo AP, Krupovic M, RNA Virus Discovery Consortium, Simmonds P, Kyrpides N, Gophna U, Dolja VV, Koonin EV.   +11 more
europepmc   +1 more source