Results 181 to 190 of about 18,532 (207)

Paralytic and Plasmatocyte Spreading Peptide From Manduca sexta

2001
A family of hemolymph peptides with diverse biological activities has been identified in several Lepidopteran species. These peptides can cause rapid paralysis (Skinner et al., 1991), block growth and development (Hayakawa, 1991), and stimulate plasmatocyte spreading and aggregation (Clark et al., 1997). The 23-residue peptides are produced from larger
Y, Wang, H, Jiang, M R, Kanost
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Uric acid excretion in larval Manduca sexta

Journal of Insect Physiology, 1980
Abstract The characteristic coating of frass of last-instar tobacco hornworms, Manduca sexta, reared on artificial diet, proved to be uric acid. Results indicated that uric acid is the major nitrogenous excretory product; during most of the larval feeding stage, 5–7% of the excreta (dry weight basis) was uric acid; only minute quantities of allantoic
J.S. Buckner, J.M. Caldwell, J.P. Reinecke   +2 more
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Intracellular calcium in prothoracic glands of Manduca sexta

Journal of Insect Physiology, 1998
Cytosolic free calcium was measured in individual prothoracic gland cells of Manduca larvae with Fura-2. During the last larval instar there was no correlation between intracellular calcium concentration and ecdysteroid secretion by the glands. The addition of prothoracicotropic hormone (PTTH) from brains of Manduca larvae to prothoracic glands in ...
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Biophysical Characterization Of An Insect Lysozyme From Manduca Sexta

Protein & Peptide Letters, 2004
Insect lysozyme from Manduca sexta (MS-lys) was overexpressed in E. coli and refolded to obtain active protein. Recombinant MS-lys presented a globular structure, with an alpha-helical content of 57% as assessed by circular dichroism spectroscopy. Light scattering studies showed that in solution MS-lys has a quasi-monodisperse size distribution, with a
Alonso A, López-Zavala, Enrique, de-la-Re-Vega, Sergio A, Calderón-Arredondo, Karina D, García-Orozco, Enrique F, Velázquez, Maria A, Islas-Osuna, Miguel A, Valdez, Rogerio R, Sotelo-Mundo   +7 more
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Ectonucleotide Diphosphohydrolase Activities in Hemocytes of Larval Manduca sexta

Archives of Biochemistry and Biophysics, 2000
In this work, we describe the ability of living hemocytes from an insect (Manduca sexta, Lepidoptera) to hydrolyze extracellular ATP. In these intact cells, there was a low level of ATP hydrolysis in the absence of any divalent metal (8.24 +/- 0.94 nmol of Pi/h x 10(6) cells).
J R, Meyer-Fernandes, H, Lanz-Mendoza, K C, Gondim, E, Willott, M A, Wells   +4 more
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Ontogeny of electroantennogram responses in the moth, Manduca sexta

Journal of Insect Physiology, 1976
Abstract The antennae of the moth, Manduca sexta , and the sensilla and sensory neurons they contain, develop during metamorphosis from pupa to adult. To determine when, during their development, antennae become capable of generating electrical responses to various stimuli, we recorded the electroantennogram (EAG), believed to be the summed ...
E S, Schweitzer, J R, Sanes, J G, Hildebrand   +2 more
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Primary culture of antennal mechanoreceptor neurons of Manduca sexta

Cell and Tissue Research, 1999
We have developed a primary cell culture system of antennal mechanoreceptor neurons from early-stage pupal sphinx moth Manduca sexta. Dissociated neurons from the moth antennae differentiated, grew and survived for several weeks in a conditioned culture medium.
P H, Torkkeli, A S, French
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Olfactory-based discrimination learning in the moth, Manduca sexta

Journal of Insect Physiology, 2001
Moths possess highly tuned olfactory capabilities, which can detect very low concentrations of pheromonal odorants. Much is known about the structure and function of the moth olfactory system with respect to detection of pheromones. However, we lack an understanding of the broader olfactory system, in particular, to what degree are moths capable of ...
K C, Daly, M L, Durtschi, B H, Smith
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Messenger RNA in the cellular commitment of Manduca sexta

General and Comparative Endocrinology, 1981
Abstract RNA synthesized during the ecdysteroid-induced change of epidermal commitment in the tobacco hornworm has been investigated. Larvally and pupally committed cells do not show any qualitative differences in total RNA that can be detected on 7 M urea polyacrylamide gels.
A C, Chen, L M, Riddiford
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Four serine proteinases expressed in Manduca sexta haemocytes

Insect Molecular Biology, 1999
Abstract Several putative serine proteinases were detected in Manduca sexta larval plasma by labelling with radio‐active diisopropyl fluorophosphate. To begin to identify and characterize such enzymes, a polymerase chain reaction was carried out using haemocyte cDNA as ...
H, Jiang, Y, Wang, M R, Kanost
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