Results 41 to 50 of about 161,880 (308)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

A Mathnavî dated 1386 among the Manucripts of Hacı Selim Ağa: Bibliophile Bureaucrats and Families

open access: yesSanat Tarihi Yıllığı, 2022
Research on manuscripts preserved in Turkish museums and libraries have extanded our knowledge of bibliophiles in the states eatablished in Anatolia between the 12th and 14th centuries, and subsequently in the Ottoman Empire, where Ottoman sultans and ...
Zeren Tanındı
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Evidências de Patrocínio em Bestiários Medievais

open access: yesMedievalista, 2021
The present article is part of an ongoing Master’s degree research in Universidade de Brasília (UnB), and proposes to indicate visual evidence of patronage in two British medieval bestiaries of the second family, Bodley Ashmole Ms.
Tiago de Oliveira Veloso Silva
doaj   +1 more source

Impact of Asymptomatic Intracranial Hemorrhage on Outcome After Endovascular Stroke Treatment

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Background Endovascular treatment (EVT) achieves high rates of recanalization in acute large‐vessel occlusion (LVO) stroke, but functional recovery remains heterogeneous. While symptomatic intracranial hemorrhage (sICH) has been well studied, the prognostic impact of asymptomatic intracranial hemorrhage (aICH) after EVT is less certain ...
Shihai Yang   +22 more
wiley   +1 more source

Bestiary Imagery in Hebrew Manuscripts of the Thirteenth Century

open access: yesReligions
In medieval bestiaries, knowledge about animals and their behavior is regularly given a Christian moral interpretation. This article explores the use of imagery related to the bestiary tradition in three Hebrew books made around the year 1300, focusing ...
Adam S. Cohen
doaj   +1 more source

The Role of Manuscript Illumination in the Chanson de geste Aiol

open access: yes, 1996
Obergfell Sandra C. The Role of Manuscript Illumination in the Chanson de geste Aiol. In: Romania, tome 114 n°455-456, 1996. pp.
Obergfell, Sandra C.
core   +1 more source

Colour Theory, Optics and Manuscript Illumination

open access: yes, 2023
Perspectiva’s synthesis of Greco-Roman, Christian and Arabic theories of light, colour and vision occasioned a revolution in European optics. It is inconceivable that illuminators working in courtly, scholarly or ecclesiastical milieu would have remained

core   +1 more source

Subclinical Optic Nerve Involvement in Radiologically Isolated Syndrome: Multimodal Detection and Diagnostic Impact

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objectives We aimed to determine the frequency of subclinical optic nerve (ON) lesions using MRI, optical coherence tomography (OCT), and visual evoked potentials (VEP) in radiologically isolated syndrome (RIS), and to assess their diagnostic and prognostic significance.
Christine Lebrun‐Frenay   +13 more
wiley   +1 more source

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