Results 201 to 210 of about 6,491,673 (269)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Domain associated with zinc fingers‐containing NF90‐NF45 complex inhibits m6A modification of primary microRNA by suppressing METTL3/14 activity

FEBS Open Bio, EarlyView.
NF90–NF45 functions as a negative regulator of methyltransferase‐like 3/14 (METTL3/14)‐mediated N6‐methyladenosine (m6A) modification on primary microRNAs (pri‐miRNAs). NF90–NF45 binds to anti‐oncogenic pri‐miRNAs and inhibits their m6A modification, thereby suppressing the biogenesis of anti‐oncogenic miRNAs.
Takuma Higuchi, Shunsuke Morioka, Keiko Morisawa, Kazutsugu Matsukawa, Shingo Ashida, Takeshi Suzuki, Shuji Sakamoto   +6 more
wiley   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Enzymatic degradation of biopolymers in amorphous and molten states: mechanisms and applications

FEBS Open Bio, EarlyView.
This review explains how polymer morphology and thermal state shape enzymatic degradation pathways, comparing amorphous and molten biopolymer structures. By integrating structure–reactivity principles with insights from thermodynamics and enzyme engineering, it highlights mechanisms that enable efficient polymer breakdown.
Anđela Pustak, Aleksandra Maršavelski
wiley   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

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Movement-aware map construction

International Journal of Geographical Information Science, 2021
Map construction algorithms attempt to derive a spatial graph representing a road network from GPS-sampled movement trajectories.
Haiyang Lyu, Dieter Pfoser, Yehua Sheng
openaire   +2 more sources

MapTRv2: An End-to-End Framework for Online Vectorized HD Map Construction

International Journal of Computer Vision, 2023
High-definition (HD) map provides abundant and precise static environmental information of the driving scene, serving as a fundamental and indispensable component for planning in autonomous driving system. In this paper, we present MapTRansformer, an end-
Bencheng Liao, Shaoyu Chen, Yunchi Zhang, Bo Jiang, Qian Zhang, Wenyu Liu, Chang Huang, Xinggang Wang   +7 more
semanticscholar   +1 more source

PivotNet: Vectorized Pivot Learning for End-to-end HD Map Construction

IEEE International Conference on Computer Vision, 2023
Vectorized high-definition map online construction has garnered considerable attention in the field of autonomous driving research. Most existing approaches model changeable map elements using a fixed number of points, or predict local maps in a two ...
Wenjie Ding, Limeng Qiao, Xi Qiu, Chi Zhang   +3 more
semanticscholar   +1 more source

Complementing Onboard Sensors with Satellite Maps: A New Perspective for HD Map Construction

IEEE International Conference on Robotics and Automation, 2023
High-definition (HD) maps play a crucial role in autonomous driving systems. Recent methods have attempted to construct HD maps in real-time using vehicle onboard sensors.
Wenjie Gao, Jiawei Fu, Haodong Jing, Nanning Zheng   +3 more
semanticscholar   +1 more source

Online Vectorized HD Map Construction using Geometry

European Conference on Computer Vision, 2023
The construction of online vectorized High-Definition (HD) maps is critical for downstream prediction and planning. Recent efforts have built strong baselines for this task, however, shapes and relations of instances in urban road systems are still under-
Zhixin Zhang, Yiyuan Zhang, Xiaohan Ding, Fusheng Jin, Xiangyu Yue   +4 more
semanticscholar   +1 more source