Results 261 to 270 of about 5,837,905 (341)

Harnessing cell aggregates for enhanced adeno‐associated virus manufacturing: Cultivation strategies and scale‐up considerations

open access: yesBiotechnology Progress, EarlyView.
Abstract The possibility to produce recombinant adeno‐associated virus (rAAV) by adherent HEK293T cells was studied in a stirred tank bioreactor (STR) culture of cell aggregates. A proof‐of‐concept of rAAV production was successfully demonstrated in a process where single cells were first expanded, then cell aggregates were formed by dilution into a ...
Brian Ladd   +2 more
wiley   +1 more source

Investigation of ammonium polyphosphate dilution with ground eggshells and lignin through the study of natural fibre composite flammability

open access: yesFire and Materials, Volume 47, Issue 1, Page 28-50, January/February 2023., 2023
Summary The interactions of Calcium carbonate (e.g., eggshell powder) and Lignin with ammonium polyphosphate (APP) when used as fire retardants were investigated. Three mixing ratios ‐ 1:3, 1:1, and 3:1, were used with natural fibre reinforced composites containing a hemp mat and an epoxy matrix manufactured using a light resin transfer moulding (L‐RTM)
Asanka P. Basnayake   +4 more
wiley   +1 more source

Improving outcomes in intensified processing via optimization of the cell line development workflow

open access: yesBiotechnology Progress, EarlyView.
Abstract As the industry continues to explore the benefits of continuous and intensified manufacturing, it is important to assure that the cell line development (CLD) workflows in practice today are well suited to generate clones that meet the unique challenges associated with these processes. Most cell lines used in intensified processes are currently
Vincent Balassi   +8 more
wiley   +1 more source

Enhancement of D‐mannitol production by fine‐tuned expression of mannitol‐1‐phosphate dehydrogenase in Synechocystis sp. PCC6803

open access: yesBiotechnology Progress, EarlyView.
Abstract D‐Mannitol production was achieved in freshwater Synechocystis sp. PCC6803 via the heterologous expression of mannitol‐1‐phosphate dehydrogenase (mtlD) and mannitol‐1‐phosphatase (m1p) under control of the strong promoter Ptrc1. However, only 5.54 mg L−1 of mannitol was found extracellularly after 7 days of cultivation, likely due to ...
Wenyang Wu   +3 more
wiley   +1 more source

Green Loyalty Programmes: Customer Trait Reactance and Reward Preferences

open access: yesJournal of Consumer Behaviour, EarlyView.
ABSTRACT Loyalty programmes (LPs, thereafter) can restrict customers' actions where they require customers to undertake specific activities (i.e., LP efforts) to collect reward points. As a consequence, these activities inevitably limit customers' future consumption freedom.
Jingxi Huang   +3 more
wiley   +1 more source

Phytochemistry, Antibacterial and Antioxidant Activities of Grewia lasiocarpa E. Mey. Ex Harv. Fungal Endophytes: A Computational and Experimental Validation Study

open access: yesChemistry &Biodiversity, EarlyView.
Schematics workflow for the isolation and characterisation of endophytic fungi from grewia lasiocarpa and their biological activity. Abstract The genus Grewia are well‐known for their medicinal properties and are widely used in traditional remedies due to their rich phytochemical composition and potential health benefits.
Nneka Augustina Akwu   +7 more
wiley   +1 more source

Cold‐Adapted Lipid A from Polaribacter sp. SM1127: A Study of Structural Heterogeneity and Immunostimulatory Properties

open access: yesChemBioChem, EarlyView.
This analysis reveals structural heterogeneity in the lipopolysaccharide lipid A from the cold‐adapted Gram‐negative bacterium Polaribacter sp. SM1127, with variations in fatty acid chain number, length, branching, saturation, and hydroxylation degree, likely aiding bacterial adaptation to cold environmental stressors. Lipopolysaccharide is shown to be
Roberta Cirella   +9 more
wiley   +1 more source

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