Results 131 to 140 of about 5,476,582 (407)

Pharmacological effects of osimertinib on a chicken chorioallantoic membrane xenograft model with the EGFR exon‐19‐deleted advanced NSCLC mutation

FEBS Open Bio, EarlyView.
Osimertinib reduces angiogenesis and PDL1 expression in in ovo tumors, transforming them into ‘cold tumors’ with lower immune activity. Anatomopathological and transcriptomic analyses highlight its therapeutic impact on tumor biology. This study underscores osimertinib's potential to reshape the tumor microenvironment and provides insights into its ...
David Barthélémy, Arnaud Vigneron, Xavier Rousset, Jérome Guitton, Emmanuel Grolleau, Margaux Raffin, Julie Balandier, Gaëlle Lescuyer, Mathilde Bardou, Florence Geiguer, Sébastien Couraud, Claire Bardel, Jean Viallet, Nazim Benzerdjeb, Léa Payen   +14 more
wiley   +1 more source

Best practices and benchmarks for intact protein analysis for top-down mass spectrometry

Nature Methods, 2019
One gene can give rise to many functionally distinct proteoforms, each of which has a characteristic molecular mass. Top-down mass spectrometry enables the analysis of intact proteins and proteoforms.
Daniel P. Donnelly, C. Rawlins, Caroline J. DeHart, L. Fornelli, Luis F. Schachner, Ziqing Lin, Jennifer L. Lippens, Krishna C. Aluri, Richa Sarin, Bifan Chen, Carter Lantz, Wonhyeuk Jung, Kendall R Johnson, A. Koller, J. Wolff, I. Campuzano, Jared R. Auclair, A. Ivanov, J. Whitelegge, L. Paša-Tolić, J. Chamot-Rooke, P. Danis, Lloyd M. Smith, Y. O. Tsybin, J. Loo, Ying Ge, N. Kelleher, J. Agar   +27 more
semanticscholar   +1 more source

Identification of inhibitors of the Salmonella FraB deglycase, a drug target

FEBS Open Bio, EarlyView.
A high‐throughput screen was used to identify inhibitors of Salmonella FraB, a drug target. Characterization of top hits (identified after an additional counter screen) revealed that some triazolidines, thiadiazolidines, and triazolothiadiazoles are mixed‐type inhibitors of FraB.
Jamison D. Law, Yuan Gao, Sravya Kovvali, Pankajavalli Thirugnanasambantham, Vicki H. Wysocki, Brian M. M. Ahmer, Venkat Gopalan   +6 more
wiley   +1 more source

QSRR Automator: A Tool for Automating Retention Time Prediction in Lipidomics and Metabolomics

Metabolites, 2020
The use of retention time is often critical for the identification of compounds in metabolomic and lipidomic studies. Standards are frequently unavailable for the retention time measurement of many metabolites, thus the ability to predict retention time ...
Bradley C. Naylor, J. Leon Catrow, J. Alan Maschek, James E. Cox   +3 more
doaj   +1 more source

Unsupervised machine learning for exploratory data analysis in imaging mass spectrometry

Mass spectrometry reviews (Print), 2019
Imaging mass spectrometry (IMS) is a rapidly advancing molecular imaging modality that can map the spatial distribution of molecules with high chemical specificity.
N. Verbeeck, R. Caprioli, Raf Van de Plas   +2 more
semanticscholar   +1 more source

Mapping Hsp104 interactions using cross‐linking mass spectrometry

FEBS Open Bio, EarlyView.
This study examines how cross‐linking mass spectrometry can be utilized to analyze ATP‐induced conformational changes in Hsp104 and its interactions with substrates. We developed an analytical pipeline to distinguish between intra‐ and inter‐subunit contacts within the hexameric homo‐oligomer and discovered contacts between Hsp104 and a selected ...
Kinga Westphal, Karolina Michalska, Andrzej Joachimiak, Lukasz A. Joachimiak   +3 more
wiley   +1 more source

Alkane biosynthesis gene expression and its increased production in recombinant cyanobacteria

FEBS Open Bio, EarlyView.
The transcription and mRNA structures of the alkane biosynthesis genes ado/aar were analysed in the representative cyanobacteria PCC 6803 and SK1‐2‐1, which produce C17H36 and C15H32, respectively. PCC 6803 transconjugants carrying these genes showed a 1.8‐ to 2.3‐fold increase in C17H36 production.
Misato Nagao, Takato Ozaki, Hirofumi Fukuda, Yu Kanesaki, Munehiko Asayama   +4 more
wiley   +1 more source

MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis

Frontiers in Microbiology, 2015
Currently microorganisms are best identified using 16S rRNA and 18S rRNA gene sequencing. However, in recent years matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a potential tool for microbial ...
N. Singhal, Manish Kumar, P. K. Kanaujia, J. S. Virdi   +3 more
semanticscholar   +1 more source

Enhancing transporter activity in heterologous expression systems with SAHA: a 2500‐times more potent and odorless alternative to butyrate

FEBS Open Bio, EarlyView.
Heterologous expression of membrane transporters in cultured cells is essential for functional characterization, but is sometimes limited by low activity. Our study compares the HDAC inhibitors butyrate, VPA and SAHA to enhance transport activity. We propose to replace butyrate by SAHA: it is equally effective, devoid of repulsive odor, costs less, and
Svenja Flögel, Maurice Tust, Samira Boussettaoui, Dietmar Fischer, Dirk Gründemann   +4 more
wiley   +1 more source

Carbon-carbon triple bond cleavage and reconstitution to achieve aryl amidation using nitrous acid esters

Nature Communications
C–C bond cleavage and recombination provide an efficient strategy for the modification and reconstruction of molecule structures. Herein, we present a method for achieving amidation of aryl C(sp2)–H bond through the cleavage and recombination of C–C ...
Zi-Ying Wang, Shoujun Wang, Nan-Nan Dai, Yao Xiao, Yu Zhou, Wen-Chan Tian, Dongru Sun, Qiang Li, Yong Wang, Wen-Ting Wei   +9 more
doaj   +1 more source