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Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source
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Materials, materiality, and media

Proceedings of the SIGCHI Conference on Human Factors in Computing Systems, 2013
In HCI, and especially in interaction design, the material aspect of interactions is currently emphasized. Nevertheless, it is challenging to theoretically frame the variety of digital or immaterial, and physical materials. In order to contribute to this materiality discourse, we reflect on McLuhan's work on media analysis and on Latour's Actor-Network
Verena Fuchsberger   +2 more
openaire   +2 more sources

Materiality matters---experience materials

Interactions, 2013
ACM CHI 2012 conference organized a panel titled 'Material Interactions - From Atoms and Bits to Entangled Practices'. The panel was specifically arranged to discuss assemblages of digital and physical materials and how these compositions might form and enable new experiences, as well as questions related to how we might conceptualize the ...
Mikael Wiberg   +7 more
openaire   +3 more sources

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