Results 201 to 210 of about 10,287,445 (293)

A multidimensional platform to support intestinal epithelial and immune cell co-culture. [PDF]

Front Nutr
Walker JL, Sprachman M, Welch M, Meehan-Qiu A, Kelly K, Shaughnessey E, Jenkins H, Iyer J, Cousens J, Engelke R, Hsi P, Christianson R, Wiellette E.   +12 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

A Functional TGF-β/Smad Assay for Targeted Profiling of Demineralized Bone Matrix-Derived Allograft Bioactivity. [PDF]

J Funct Biomater
Lendvai A, Weichhart T, Weitzenböck HP, Wiesner C, Seeboeck R, Zamani N, Matzner M, Pichler M, Steiner B, De Luna A, Nehrer S, Hundsberger H.   +11 more
europepmc   +1 more source

De novo synthesis of short‐chain aldehydes and hydrocarbons secreted by the brown marmorated stink bug Halyomorpha halys

FEBS Open Bio, EarlyView.
The brown marmorated stink bug, Halyomorpha halys, mainly secretes 4‐oxo‐(E)‐2‐hexenal, (E)‐2‐decenal, dodecane and tridecane, a mixture representative of that released by many heteropterans. We demonstrated that H. halys can synthesise these secretory components de novo using dietary glucose.
Haruna Fujimori, Koji Noge
wiley   +1 more source

A Scalable Bioreactor Platform for Reproducible Production and Characterization of Ovarian Cancer-Derived Extracellular Vesicles. [PDF]

Bioengineering (Basel)
Fu W, Dorayappan KDP, Hisey C, Chakrapani LN, Wiggins S, Sundaram S, Lambert Z, Nguyen KT, Chelian SA, Reategui E, Selvendiran K, Hansford DJ.   +11 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

From patient advocacy to patient‐driven research: Building active partnerships beginning at the bench to reach the bedside

FEBS Open Bio, EarlyView.
Research is strongest when conducted alongside patients, not just about them. Patient research organizations help integrate patient perspectives into research priorities, study design, and scientific meetings, leading to meaningful patient outcomes and development of relevant therapies.
Jenica H. Kakadia, Rachel Heilmann, Maximiliano Barrientos, Laura MacNeill, Ingrid Vallee, Sarah K. Schultz, Patrick O'Donoghue, Angelica A. Moresco, Victoria M. Siu, Ilka U. Heinemann   +9 more
wiley   +1 more source

Versatile twin layer macromolecular fibers for advanced tissue engineering applications. [PDF]

iScience
Taylor CS, Majd H, Gregory DA, Fricker A, Daniel Syed Mohamed SM, Hinchcliffe J, Asare E, Farr NT, Alenezi H, Welsh GI, Edirisinghe M, Roy I.   +11 more
europepmc   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

A Case of Nontuberculous Mycobacterial Prosthetic Valve Endocarditis. [PDF]

Spartan Med Res J
Esmaili S, Khan S, Restini C, Hanson C, Srivastava S, Neff D.   +5 more
europepmc   +1 more source