RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source
Green Synthesis of ZnO Nanoparticles Using <i>Ocimum basilicum</i> var. <i>purpurascens</i>: As-Synthesized Phase Formation and Thermal Evolution of Optical Properties. [PDF]
Iriqui-Razcón JL +6 more
europepmc +1 more source
Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj +3 more
wiley +1 more source
Emergence of Moiré Dirac Fermions at the Interface of Topological and 2D Magnetic Insulators. [PDF]
Klimovskikh II +12 more
europepmc +1 more source
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point +7 more
wiley +1 more source
First-principles calculation of adsorption of cadmium and lead on the surface of a 1T-MnO2 monolayer. [PDF]
Dita-Casiano JV +4 more
europepmc +1 more source
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley +1 more source
Green carbon dots-embedded polymeric membranes for simultaneous photocatalytic dye degradation and antibacterial activity. [PDF]
López Amador A +7 more
europepmc +1 more source
Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada +5 more
wiley +1 more source
A Spin Frustrated Hourglass <b>{Gd</b><sub><b>9</b></sub><b>}</b> Molecular Nanomagnet with Unusual Magnetocaloric Properties. [PDF]
Panguluri SPK +11 more
europepmc +1 more source

