Results 151 to 160 of about 279,481 (266)

Fluorescent organelle markers in Cryptococcus neoformans: a versatile toolkit for live-cell subcellular localization. [PDF]

Genetics
Choi Y, Bahn YS, Heitman J.
europepmc   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Sexual reproduction and the polygenic architecture of azole resistance in agricultural populations of <i>Aspergillus fumigatus</i>. [PDF]

Front Microbiol
Huang J, Liu H, He Y, Zhang Y.
europepmc   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Beyond the Black Box: Reproductive Strategies of the Black Soldier Fly as a Model for Bridging Evolutionary Biology and Applied Entomology. [PDF]

Evol Appl
Lemke NB, Puniamoorthy N.
europepmc   +1 more source

Small RNA pathways in mammalian oocytes

FEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

Transcriptional response of spermathecal secretory cells to mating and receipt of seminal fluid proteins in <i>Aedes aegypti</i>. [PDF]

iScience
Wyer CAS, Afkhami M, Ellis DA, Pitcher S, Amaro IA, Perish PK, Angelico JW, Gray EM, Ahmed-Braimah YH, Wolfner MF, Harrington LC.   +10 more
europepmc   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

Endogenous corazonin signaling modulates the post-mating switch in behavior and physiology in females of the brown planthopper and <i>Drosophila</i>. [PDF]

Elife
Zhang N, Su SC, Bu RT, Zhang Y, Yang L, Chen J, Nässel DR, Gao C, Wu SF.   +8 more
europepmc   +1 more source

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source