Results 211 to 220 of about 6,975,086 (253)

Comparison of Phenotypic MRSA Detection Methods with PCR for mecA Gene in the Background of Emergence of Oxacillin-Susceptible MRSA

Microbial Drug Resistance, 2021
Background: Phenotypic methods for detection of methicillin resistance in Staphylococcus aureus (MRSA) can be inaccurate due to heterogeneous expression of resistance and due to environmental factors that influence the expression of resistance.
D. Nair, Nandita Shashindran, Anil Kumar, Vivek Vinodh, Lalitha Biswas, Raja Biswas   +5 more
semanticscholar   +1 more source

Staphylococcus pseudintermedius heterogeneously expresses the mecA gene.

Veterinary Microbiology, 2013
V. Savini, Nicola Di Giuseppe, P. Fazii, C. D'amario, D. D’Antonio, E. Carretto   +5 more
semanticscholar   +3 more sources

mecA Gene Dissemination Among Staphylococcal and Non-staphylococcal Isolates Shed in Surface Waters

Bulletin of Environmental Contamination and Toxicology, 2015
Aquatic ecosystems represent important vehicles for the dissemination of antibiotic resistant bacteria and antibiotic resistance genes. Of particular interest are methicillin-resistant staphylococci (MRS) harboring mecA gene that confers their resistance to β-lactams. Therefore, in this study, water samples collected from different locations of a river
Elnaz Seyedmonir, Fadime Yılmaz, B. Icgen   +2 more
semanticscholar   +5 more sources

Rapid identification of bacteria, mecA and van genes from blood cultures

Pathologie Biologie, 2007
The Genotype technology, a quick molecular genetic assay based on DNA multiplex amplification with biotinylated primers followed by hybridization to membrane bound probes, complies with the requirements for a fast diagnosis of sepsis. We evaluated the new Genotype BC Gram-negative and Gram-positive test kits (Hain Life Science, Germany) which ...
M-F, Prère, O, Baron, O, Fayet
openaire   +2 more sources

Degradation Kinetics of Antibiotic Resistance Gene mecA of Methicillin-Resistant Staphylococcus aureus (MRSA) during Water Disinfection with Chlorine, Ozone, and Ultraviolet Light.

Environmental Science and Technology, 2021
Degradation kinetics of antibiotic resistance genes (ARGs) by free available chlorine (FAC), ozone (O3), and UV254 light (UV) were investigated in phosphate buffered solutions at pH 7 using a chromosomal ARG (mecA) of methicillin-resistant Staphylococcus
Yegyun Choi, Huan He, Michael C. Dodd, Yunho Lee   +3 more
semanticscholar   +1 more source

The Molecular Detection of mecA Genes of Staphylococcus Aureus

Annals of Advanced Biomedical Sciences, 2019
Antibiotic resistance is common among pathogenic bacteria associated with community acquired and nosocomial infections. Methicillin-resistant Staphylococcus aureus (MRSA) infections have become a global health problem particularly in hospital setup causing simple skin infections to life threatening infections. The present study aimed to investigate the
openaire   +1 more source

[MRSA--detection of mecA and its regulatory genes].

Rinsho byori. The Japanese journal of clinical pathology, 1994
Methicillin resistance in S.aureus and S.epidermidis strains is primarily due to production of a new penicillin-binding protein PBP2' with extremely low binding affinity to most beta-lactam antibiotics. The structural gene for PBP2', mecA, is detectable in clinical specimens by using the polymerase chain reaction (PCR).
S, Kagawa, K, Yamashita, A, Matsuoka
openaire   +1 more source

Surveillance of variant mecA gene and analysis of efflux pump in mecA negative MRSA infections.

Pakistan journal of pharmaceutical sciences, 2019
It has been known from the medical records of Kancheepuram district hospitals that there is a prevalence of Methicillin resistant Staphylococcus aureus strain. Now the recent epidemiology has shown the presence of Methicillin Resistant Staphylococcus aureus even in school going children community individuals. In view of the above situation, Methicillin
Karthikeyan, Vijayan, Sulochana, Somasundaram, Catherine, Leo, Jayaprakash, Kanagaraj   +3 more
openaire   +1 more source

Rapid Detection of the Methicillin-resistance Gene,mecA, in Coagulase-negative Staphylococci

Scandinavian Journal of Infectious Diseases, 2001
Phenotypical methods are routinely used to detect methicillin resistance in Staphylococci. These methods are time-consuming and there are difficulties in detecting all resistant strains carrying the mecA gene. We detected methicillin-resistant Staphylococci in biological samples by PCR amplification of mecA, without the time-consuming step of ...
T, Glad, C, Klingenberg, T, Flaegstad, J U, Ericson, O, Olsvik   +4 more
openaire   +2 more sources

[Combination PCR of mecA, femA genes for detection of MRSA].

Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition, 2004
To make a comparison of PCR assay, Agar Dilution and Disk Diffusion as for detecting Staphylococcal mecA, femA genes.A total of 178 strains of Staphylococci were isolated from three large scale hospitals in Chengdu. Disk Diffusion and staphylococcal mecA, femA gene PCR assay for detecting MRSA were compared with Agar Dilution.The sensitivity and ...
Zhihong, Chen, Xinjian, Fan, Xiaoju, Lü
openaire   +1 more source