Results 211 to 220 of about 20,723,232 (242)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Mobile Phone Access, Usage Patterns, and Perceptions of Adolescents Living With HIV on the Use of Gamified Interventions to Improve Antiretroviral Therapy Adherence in Eswatini: Qualitative Study. [PDF]

JMIR Mhealth Uhealth
Hlophe LD, Nyasulu PS, Shumba CS.
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

FEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima, Gustavo Narvaes Guimarães, Emilyane de Oliveira Santana Amaral, Bianca Caroline Figueiredo Bianco, Manuel Jara‐Espejo, Sérgio Roberto Peres Line, Ana Paula de Souza, Aline Cristiane Planello   +7 more
wiley   +1 more source

How do Japanese medical students use social media? A large multicenter survey of platform preferences and career decision-making. [PDF]

Surg Today
Yamamoto T, Hida K, Ishihara S, Murakami S, Yamane Y, Kinjo T, Kinoshita M, Horimukai K, Otsuka A, Takami H, Obama K.   +10 more
europepmc   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Social media toolkit for recruiting clinical research participants: A new resource from the Recruitment Innovation Center. [PDF]

J Clin Transl Sci
Cook S, Mayers S, Schorr M, Rantala C, Gomez S, Vance M, Pardue D, Edwards TL, Stroud M, Harris PA, Wilkins C.   +10 more
europepmc   +1 more source

The relationship between smartphone usage and sleep quality among university students: a cross-sectional study. [PDF]

Sci Rep
Ishak MK, Yasmin F, Islam J, Butt AUR, Alamoudi AM, Hossain MA.   +5 more
europepmc   +1 more source

The Psychometric Validity of the TikTok Addiction Scale Turkish Version: The Relationship between TikTok Addiction, Stress, and Personality. [PDF]

Sisli Etfal Hastan Tip Bul
Cokmus FP, Cayli SBD, Aydin O.
europepmc   +1 more source

The Relationship between Social Media and Schizophrenia and Other Psychotic Disorders: A Systematic Review. [PDF]

Schizophr Bull Open
Cipriani M, Notsu H, Vaidya S, Castillo J, Hau C, Li S, Xia W, Yacoub D, Zhou SZ, Birnbaum ML, Torous J.   +10 more
europepmc   +1 more source