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DNA transfer by highly asymmetric somatic hybridisation in Medicago truncatula (+) Medicago rugosa and Medicago truncatula (+) Medicago scutellata

Theoretical and Applied Genetics, 2002
A regenerable line of Medicago truncatula(Jemalong 2HA) as a recipient species, was fused with the sexually incompatible species Medicago scutellataor Medicago rugosa.The treatments described maintain the chromosome number of the recipient but enable the transfer of small amounts of DNA of the donor species, probably by intergenomic recombination ...
D, Tian, C, Niu, R J, Rose
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Micropropagation of Medicago truncatula Gaertn. cv. Jemalong and Medicago truncatula ssp. Narbonensis

Plant Cell, Tissue and Organ Culture, 2001
A simple and reliable method was established for the maintenance of a permanent stock of several Medicago truncatula genotypes selected from a general seed stock by their in vitro culture amenability and embryogenic capacity. In the first step, multiple shoots were induced from the cotyledon axillary meristem meristem of pre-germinated seeds in ...
L.O. Neves, L. Tomaz, M.P.S. Fevereiro
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Nod-Factor Perception in Medicago truncatula

2008
Nod factors in 1990 (see Denarie et al., 1996), much attention has been paid to how these intriguing molecules interact with plants and this has led to the recent identification of legume symbiotic receptor genes. These studies were aided by focussing on model systems in which genetic and genomic approaches could be used.
Cullimore, Julie Vera   +14 more
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Medicago truncatula Transformation Using Leaf Explants

2014
Legumes have been for a long time recalcitrant to efficient Agrobacterium transformation. The choice and use of model legume plants (Medicago truncatula and Lotus japonicus) for molecular studies has triggered extensive studies devoted to the development of efficient Agrobacterium-mediated transformation protocols for these two plants. In M. truncatula,
Viviane, Cosson   +2 more
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Medicago truncatula Transformation Using Cotyledonary Explants

2014
Medicago truncatula has been developed into a model species for legumes. The M. truncatula genotype Jemalong A17 has been used for EST and genome sequencing. However, this genotype is difficult to regenerate from callus cultures. By using cotyledons as explants for Agrobacterium infection and direct shoot formation, this protocol allows for rapid ...
Elane, Wright, Zeng-Yu, Wang
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Tnt1 Insertional Mutagenesis in Medicago truncatula

2018
Legumes play irreplaceable roles in sustainable agriculture due to their unique capability of fixing gaseous nitrogen in the atmosphere and turning into plant-usable ammonium through interaction with rhizobia. With the completion of genome sequencing of several model and non-model legumes, it is highly desirable to generate mutant populations for ...
Hee-Kyung, Lee   +2 more
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Annotating the genome of Medicago truncatula

Current Opinion in Plant Biology, 2006
Medicago truncatula will be among the first plant species to benefit from the completion of a whole-genome sequencing project. For each of these species, Arabidopsis, rice and now poplar and Medicago, annotation, the process of identifying gene structures and defining their functions, is essential for the research community to benefit from the sequence
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Translating Medicago truncatula genomics to crop legumes

Current Opinion in Plant Biology, 2009
Genomic resources developed in the model legume, Medicago truncatula, have the potential to accelerate practical advances in crop legumes. M. truncatula is closely related to many economically important legumes, frequently displaying genome-scale synteny. Translating genome data from M.
Nevin Dale, Young, Michael, Udvardi
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Gentisic acid conjugates of Medicago truncatula roots

Phytochemistry, 2009
Three phenolic glycosides 5-O-{[5''-O-E-(4'''-O-threo-guaiacylglycerol)-feruloyl]-beta-apiofuranosyl-(1-->2)-beta-xylopyranosyl} gentisic acid, 5-O-[(5''-O-vanilloyl)-beta-apiofuranosyl-(1-->2)-beta-xylopyranosyl] gentisic acid and 1-O-[E-(4'''-O-threo-guaiacylglycerol)-feruloyl]-3-O-beta-galacturonopyranosyl glycerol were isolated and identified from ...
STOCHMAL ANNA   +5 more
openaire   +3 more sources

Characterization of KNOX genes in Medicago truncatula

Plant Molecular Biology, 2008
We isolated three class I and three class II KNOX genes in Medicago truncatula. The predicted amino acid sequences suggested a possible orthology to the Arabidopsis homeodomain proteins STM, KNAT1/BP, KNAT3 and KNAT7 that was confirmed by phylogenetic and conserved structural domain analyses.
Elisabetta Di Giacomo   +5 more
openaire   +4 more sources

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