Practices and outcomes of multidisciplinary collaboration to support the integration of healthcare into the daily lives of technology-dependent children. [PDF]
Watanabe R, Shigematu Y.
europepmc +1 more source
CDT1 is an essential protein for DNA replication licensing that loads the MCM complex, the eukaryotic replicative DNA helicase, onto replication origins. Overexpression of CDT1 induces cell cycle arrest at the S phase. Here we showed CDT1 inhibits the progression of replication forks by interacting with the MCM complex, leading to the stalling and ...
Takashi Tsuyama +7 more
wiley +1 more source
Increasing trend in fusidic acid resistance among MRSA isolates in the Netherlands, 2016-23. [PDF]
Velthuis F +7 more
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Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley +1 more source
A student-led MD-PhD program wellness initiative. [PDF]
Skelton AM +5 more
europepmc +1 more source
Homologous expression and purification of human HAX‐1 for structural studies
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Clinical Large Language Model Evaluation by Expert Review (CLEVER): Framework Development and Validation. [PDF]
Kocaman V, Kaya MA, Feier AM, Talby D.
europepmc +1 more source
A Parent's Perspective on Systemic Gaps in Supporting Complex Pediatric Care at Home. [PDF]
Cole AC.
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ATTITUDES AND PRACTICE PATTERNS OF CANADIAN PHYSIATRISTS REGARDING MEDICAL CANNABIS. [PDF]
Ethans K +5 more
europepmc +1 more source

