Results 191 to 200 of about 4,320,334 (318)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Engineering a genetic construct to express soluble proteins tethered to the cell membrane. [PDF]

Biosci Rep
Ronco-Campaña A, Simms J, Goddard AD, Rothnie AJ.   +3 more
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Porcine diazepam-binding inhibitor and bovine diazepam-binding inhibitor affect morphine antinociception via different receptors

Protein & Cell, 2016
Yu-Zhen Chen, Xiao-Cun Li, Zhen-Quan Guo, Li Zhou, Zhuan Zhou, Song-Ping Liang, Cai-Hong Wu   +6 more
doaj   +1 more source

An Unbiased Geometry-Resolved Membrane Platform for Proteome-Scale Discovery of Membrane Curvature Sensors. [PDF]

Adv Sci (Weinh)
Komikawa T, Kawakami R, Chattrairat K, Niwa T, Ajiri T, Yasui T, Tanaka M.   +6 more
europepmc   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Membrane affinity difference between MinD monomer and dimer is not crucial for MinD gradient formation in <i>Bacillus subtilis</i>. [PDF]

Elife
Bohorquez LC, Strahl H, Marenduzzo D, Thiele MJ, Burmann F, Hamoen L.   +5 more
europepmc   +1 more source

On Membranes and Plates [PDF]

Proceedings of the National Academy of Sciences, 1950
openaire   +2 more sources

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Regulation of Nanomaterials on the Preparation of Nanofiltration Membranes and Its Research Progress in the Water Treatment Field: A Review. [PDF]

Water Environ Res
Quan R, Hao X, Hu Y, Xu X, Liu X, Zhang X, Yun X.   +6 more
europepmc   +1 more source