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Membrane Protein Solubilization [PDF]

, 2009
A critical step in any in vitro analysis of membrane proteins is the solubilization of the membrane to extract the protein of interest in an active form to obtain an aqueous solution containing the membrane protein complexed with detergents and lipids in a form suitable for purification and further analysis. This process is particularly delicate as the
Katia Duquesne, James N. Sturgis
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Membrane Protein Simulations Using AMBER Force Field and Berger Lipid Parameters.

Journal of Chemical Theory and Computation, 2012
AMBER force fields are among the most commonly used in molecular dynamics (MD) simulations of proteins. Unfortunately, they lack a specific set of lipid parameters, thus limiting its use in membrane protein simulations.
A. Cordomí, G. Caltabiano, L. Pardo
semanticscholar   +1 more source

Membrane-protein engineering

Trends in Biotechnology, 1997
Membrane proteins perform many of the essential functions required for life. They are often the targets of medicinal drugs and have many potential uses in biotechnological processes. Therefore our ability to understand them and manipulate their functions is both important and necessary to enable protein engineers to create 'designer' membrane proteins (
G. von Heijne, P. Whitley, Ismael Mingarro   +2 more
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Ubiquitin and membrane protein turnover: from cradle to grave.

Annual Review of Biochemistry, 2012
From the moment of cotranslational insertion into the lipid bilayer of the endoplasmic reticulum (ER), newly synthesized integral membrane proteins are subject to a complex series of sorting, trafficking, quality control, and quality maintenance systems.
J. MacGurn, Pi-Chiang Hsu, S. Emr
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Crystallization of membrane proteins

Trends in Biochemical Sciences, 1983
Abstract After a long period of fruitless attempts, three-dimensional crystal of membrane proteins are now available. The best of the crystals are suited for high-resolution structure determination by X-ray crystallography. The use of small detergents and small amphiphilic molecules during crystallization proved to be of great value.
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Bioinformatics of Membrane Proteins

2002
As for all proteins, there are three basic problems facing bioinformatics in the field of membrane proteins: to reliably identify membrane proteins in genome-wide data sets, to predict as much as possible about their structure, and to predict as much as possible about their function.
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Crystallization of Membrane Proteins

2013
The crystallization of membrane proteins is an essential technique for the determination of atomic models of three-dimensional structures by X-ray crystallography. The compositions of solutions of purified membrane proteins are altered, so as to transiently induce supersaturation, a requirement for crystal nucleation and growth.
Florian Müller, C. Roy D. Lancaster
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Membrane proteins Membrane protein structure

Current Opinion in Structural Biology, 1996
John E. Walker, Matti Saraste
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Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death

Nature, 1990
D. Hockenbery, G. Núñez, C. Milliman, R. Schreiber, S. Korsmeyer   +4 more
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Structures of membrane proteins

The Journal of Membrane Biology, 1978
The possible conformations of integral membrane proteins are restricted by the nature of their environment. In order to satisfy the requirement of maximum hydrogen bonding, those portions of the polypeptide chain which are in contact with lipid hydrocarbon must be organized into regions of regular secondary structure.
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