Results 301 to 310 of about 4,887,906 (384)

Biophysical characterization and ion transport with cell‐based and proteoliposome reconstitution assays of invertebrate K+‐Cl− cotransporters

FEBS Open Bio, EarlyView.
The K+‐Cl− cotransporters (KCCs) facilitate the symport of ions across the plasma membrane. They participate in physiological processes including neuronal regulation. Here, we characterized KCCs from Drosophila and Hydra vulgaris. Comparative analyses of transporters provide insights into the mechanism of KCC ion transport, regulation, and evolution ...
Satoshi Fudo, Marina Verkhovskaya, Coralie Di Scala, Claudio Rivera, Tommi Kajander   +4 more
wiley   +1 more source

Recent Advances on the Positively-Charged Nanofiltration Membranes for Mg²⁺/Li⁺ Separation Through Interfacial Polymerization. [PDF]

Nanomaterials (Basel)
Zeng X, Meng C, Xu Z, Li X, Zhu H, Li G.
europepmc   +1 more source

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

FEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source

Fabrication of a novel porous silicon biomembrane for applications in organ-on-chip technology. [PDF]

Biomed Microdevices
Williams MAC, Wiens C, Genc S, Thompson S, Gislason L, Blake D, Jessing J.   +6 more
europepmc   +1 more source

Analysis of the regulation of undecaprenyl diphosphate dephosphorylation in Escherichia coli

FEBS Open Bio, EarlyView.
BacA, PgpB, and YbjG phosphatases are involved in undecaprenyl phosphate (C55P) synthesis in Escherichia coli. We analyzed the lipid contents and the gene expression in the gene‐disruption strains. Undecaprenyl diphosphate (C55PP) level increased in the bacA, ybjG double‐disruption strain, but C55P levels were similar in all strains.
Tomotaka Jitsukawa, Yasushi Shigeri, Shingo Fujisaki   +2 more
wiley   +1 more source

Amphipathic helical peptide-Nile red probes for fluorescence probing of the lipid packing defects and their surrounding membranes on exosomes. [PDF]

Sci Rep
Sato Y, Segawa K, Sakamoto T, Narita A, Matsumoto K, Nishizawa S.   +5 more
europepmc   +1 more source

Functional parameters of spermatozoa obtained by a new selection device

FEBS Open Bio, EarlyView.
This study compares density gradient centrifugation and the LensHooke CA0™ device for sperm separation. DGC requires multiple steps, specialized equipment, and technical expertise. In contrast, the CA0 device offers key practical and methodological advantages, resulting in a faster, simpler, and more affordable sperm selection method.
Julio C. Chávez, Paulina Torres, Gabriela Carrasquel‐Martínez, María G. Figueroa‐Méndez, Diana Flores, Lina Villar, Israel Maldonado, Marcela B. Trevino, Claudia Treviño   +8 more
wiley   +1 more source

Characterization of Polylactic Acid Membranes for Local Release of Tramadol. [PDF]

Int J Mol Sci
Fernández-Minotre L, Montero-Aguilar M, Vázquez-Vázquez FC, Serrano-Bello J, Vega-Baudrit J, Pereira-Reyes R, Pozos-Guillén A, Chavarría-Bolaños D.   +7 more
europepmc   +1 more source

Matrix: a complex amalgam of structures and functions in tumor microenvironment

FEBS Open Bio, EarlyView.
The matrix is a dynamic, intricate three‐dimensional mesh of biomolecules with both structural and functional properties. This review deals with the complexity of this ‘molecular amalgam’ in the tumor microenvironment and highlights its importance in the maintenance and evolution of tumors by describing certain matrix biomolecules, such as ...
Spyros S. Skandalis, Evgenia Tsoukala, Theodora D. Sarantopoulou, Maria‐Elpida Christopoulou   +3 more
wiley   +1 more source

Amphiphilic nanowire-assisted monomer shuttling enables ultra-selective reverse osmosis membranes for water purification. [PDF]

Nat Commun
Zhou H, Zhang X, Li Z, Qiu Z, Yang Z, Dai R, Wang Z.   +6 more
europepmc   +1 more source