Optimizing the lysis step in CTAB DNA extractions of silica-dried and herbarium leaf tissues. [PDF]
Abstract Premise The use of cetyltrimethylammonium bromide (CTAB) is an effective and inexpensive method of extracting DNA from plants. The CTAB protocol is frequently modified to optimize DNA extractions, but experimental approaches rarely perturb a single variable at a time to systematically infer their effect on DNA quantity and quality. Methods and
Carey SJ +3 more
europepmc +2 more sources
Trust your guts? The effect of gut section on diet composition and impact of Mus musculus on islands using metabarcoding. [PDF]
In this article, we assessed the differences in diet estimates and potential ecological and economic impact measurements according to the section of the gastrointestinal tract (stomach, small and large intestine) analysed. We aimed to test which section(s) provided a better diet representation and putative impact of a common inland invader.
Pinho CJ +5 more
europepmc +2 more sources
Incomplete recovery of seeds from scatterhoards by granivorous rodents: Implications for plant establishment. [PDF]
We documented the sloppiness of seed removal from scatterhoards of soapweed yucca (Yucca glauca) seeds by Ord's kangaroo rats (Dipodomys ordii). In two different experiments, we observed that >50% of caches removed or pilfered by kangaroo rats in the Sandhill Region of Nebraska had at least one seed remaining, with about 30% leaving limited seeds at ...
Geluso K, Longo PC, Harner MJ, White JA.
europepmc +2 more sources
A New Species of Mentzelia (Loasaceae) from Mono County, California
Abstract A new species Mentzelia monoensis Brokaw and Hufford, endemic to Mono County, California, is described. Mentzelia monoensis, a hexaploid, is most similar to two widespread species, M. montana (Davidson) Davidson (tetraploid) and M. albicaulis (Douglas ex Hook.) Douglas ex Torr. & A. Gray (octoploid).
Larry Hufford
exaly +3 more sources
Comprehending Cornales: phylogenetic reconstruction of the order using the Angiosperms353 probe set. [PDF]
Premise Cornales is an order of flowering plants containing ecologically and horticulturally important families, including Cornaceae (dogwoods) and Hydrangeaceae (hydrangeas), among others. While many relationships in Cornales are strongly supported by previous studies, some uncertainty remains with regards to the placement of Hydrostachyaceae and to ...
Thomas SK +7 more
europepmc +2 more sources
Comparative diversification analyses of Hydrangeaceae and Loasaceae reveal complex evolutionary history as species disperse out of Mesoamerica. [PDF]
Abstract Premise The movement of lineages into novel areas can promote ecological opportunity and adaptive radiation, leading to significant species diversity. Not all studies, however, have identified support for ecological opportunity associated with novel intercontinental colonizations. To gain key insights into the drivers of ecological opportunity,
Schenk JJ, Jacobs S, Hufford L.
europepmc +2 more sources
Vegetation state changes in the course of shrub encroachment in an African savanna since about 1850 CE and their potential drivers. [PDF]
We present the first multidecadal time series with high taxonomic and temporal resolution, which includes sedaDNA, and that enables the tracking of vegetation and environmental change in a southern African savanna. According to our results, shrub encroachment occurs on a scale of one century and with a phase of transition characterized by a steady ...
Tabares X +9 more
europepmc +2 more sources
FUNCTION OF MENTZELIA NUDA (LOASACEAE) POSTFLORAL NECTARIES IN SEED DEFENSE
Mentzelia nuda is a coarse, short‐lived perennial of the High Plains of North America. The flowers secrete nectar which serves as an attractant for pollinating bees. Postfloral nectar secretion attracts ants whose presence significantly enhances seed set. This is the first demonstration of advantage to postflowering activity of a floral nectary.
exaly +3 more sources
Abstract Soil specialists can have restricted distributions, and effective management in the face of land use change depends on a thorough understanding of the ecology of these unique plants. We investigated the ecology of Eriogonum tiehmii Reveal, a rare soil specialist, using pitfall traps, flower observations, and pollinator exclusion to assess ...
Jamey D. McClinton +2 more
wiley +1 more source
Community‐level assisted migration for climate‐appropriate prairie restoration
Climate change threatens the long‐term survival of highly fragmented habitats such as North American tallgrass prairie. De novo plantings into former agricultural sites can contribute to efforts to conserve prairie biodiversity, but past planting practice (including our own) has focused on the use of local species and genotypes.
Mark J. McKone, Daniel L. Hernández
wiley +1 more source

