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Methodological trends and perspectives of animal dietary studies by noninvasive fecal DNA metabarcoding

Environmental DNA, 2020
Fine‐scale estimation of trophic interactions is an important subject in the field of ecology. Diet analysis based on fecal DNA metabarcoding has been accepted as a noninvasive, accurate, and time‐ and cost‐effective tool to determine animal diets. Here,
Haruko Ando, Haruka Mukai, Taketo Komura   +2 more
exaly   +2 more sources

Comparison of environmental DNA metabarcoding and bottom trawling for detecting seasonal fish communities and habitat preference in a highly disturbed estuary

Ecological Indicators, 2023
Environmental DNA (eDNA) metabarcoding has been used to study fish diversity in various aquatic ecosystems. However, studies on fish community structure in estuarine ecosystems have not been well corroborated by eDNA metabarcoding, and comparisons with ...
Peiwen Jiang, Shannan Xu, Zuozhi Chen
exaly   +3 more sources

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Metabarcoding of Litter-Associated Fungi and Bacteria

2020
Litter-associated microorganisms play key roles in forested streams by decomposing and transferring energy from plant litter to higher trophic levels. To circumvent the shortcomings of traditional microbiological methods and conventional microscopy to assess the composition of microbial communities associated with decomposing litter, a range of DNA ...
Duarte, Sofia Alexandra Ferreira, Wurzbacher, Christian, Seena, Sahadevan   +2 more
openaire   +2 more sources

IRP: eDNA metabarcoding

2022
Documents and data supporting an undergraduate independent research project final report about the effectiveness of eDNA metabarcoding as a mammal monitoring ...
openaire   +1 more source

Metabarcoding in gut protozoology

Trends in Parasitology
Next-generation sequencing (NGS) methods include whole-genome sequencing, metagenomic analysis, and amplicon-based NGS, all of which are gaining territory in parasitology. A modality of particular interest within the field of gut protozoology is exhaustive metabarcoding of ribosomal genes in a complex matrix such as faeces, by which method, amplicon ...
openaire   +2 more sources

The future of eDNA metabarcoding

2018
Abstract Environmental DNA-based research is undergoing rapid developments, but its democratization in basic and applied research remains hampered by the biases introduced by molecular approaches, the difficulties in estimating absolute organisms’ abundances, and a lack of general consensus in molecular protocols.
Pierre Taberlet, Aurélie Bonin, Lucie Zinger, Eric Coissac   +3 more
openaire   +1 more source

DNA Metabarcoding: Current Applications and Challenges

Journal of Agricultural and Food Chemistry
DNA metabarcoding is a versatile approach that combines high-throughput sequencing (HTS) with DNA barcoding and enables the simultaneous identification of multiple species in complex DNA samples. With the rapid development of high-throughput sequencing (HTS), DNA meta-barcoding has been widely applied in many fields.
Huilin Yuan, Jianping Han, Meihua Yang, Shilin Chen, Xiaohui Pang   +4 more
openaire   +2 more sources

DNA metabarcode choice and design

2018
Abstract Chapter “DNA metabarcode choice and design” develops the properties of the ideal metabarcode in a given context, including conservation of the primer annealing regions and resolution power across the target taxonomic group of interest.
Pierre Taberlet, Aurélie Bonin, Lucie Zinger, Eric Coissac   +3 more
openaire   +1 more source