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Toward quantitative metabarcoding
Ecology, 2022AbstractAmplicon-sequence data from environmental DNA (eDNA) and microbiome studies provides important information for ecology, conservation, management, and health. At present, amplicon-sequencing studies – known also as metabarcoding studies, in which the primary data consist of targeted, amplified fragments of DNA sequenced from many taxa in a ...
Andrew Olaf Shelton +10 more
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2022
Documents and data supporting an undergraduate independent research project final report about the effectiveness of eDNA metabarcoding as a mammal monitoring ...
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Documents and data supporting an undergraduate independent research project final report about the effectiveness of eDNA metabarcoding as a mammal monitoring ...
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Metabarcoding in gut protozoology
Trends in ParasitologyNext-generation sequencing (NGS) methods include whole-genome sequencing, metagenomic analysis, and amplicon-based NGS, all of which are gaining territory in parasitology. A modality of particular interest within the field of gut protozoology is exhaustive metabarcoding of ribosomal genes in a complex matrix such as faeces, by which method, amplicon ...
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DNA metabarcoding data analysis
2018Abstract DNA metabarcoding generates huge amounts of data containing noise introduced by molecular methods. Chapter 8 “DNA metabarcoding data analysis” discusses the analytic steps and available software to curate and evaluate DNA metabarcoding data prior to final ecological analyses. It provides command lines to perform primary analyses
Pierre Taberlet +3 more
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Enhancing amphibian biomonitoring through eDNA metabarcoding
Molecular Ecology ResourcesAbstractSurveying biodiversity has taken a quantum leap with environmental DNA (eDNA) metabarcoding, an immensely powerful approach lauded for its efficiency, sensitivity, and non‐invasiveness. This approach emerges as a game‐changer for the elusive realm of endangered and rare species—think nocturnal, environmentally elusive amphibians.
Yawen Mu +6 more
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Metabarcoding of Litter-Associated Fungi and Bacteria
2020Litter-associated microorganisms play key roles in forested streams by decomposing and transferring energy from plant litter to higher trophic levels. To circumvent the shortcomings of traditional microbiological methods and conventional microscopy to assess the composition of microbial communities associated with decomposing litter, a range of DNA ...
Duarte, Sofia Alexandra Ferreira +2 more
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The future of eDNA metabarcoding
2018Abstract Environmental DNA-based research is undergoing rapid developments, but its democratization in basic and applied research remains hampered by the biases introduced by molecular approaches, the difficulties in estimating absolute organisms’ abundances, and a lack of general consensus in molecular protocols.
Pierre Taberlet +3 more
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Lebensmittel‐Authentizitätsprüfung mittels NGS‐Metabarcoding
LebensmittelchemieDie Authentizitätsprüfungen von Lebensmitteln und deren Rohstoffen ist in der amtlichen Lebensmittelüberwachung schon seit einiger Zeit ein großes Themenfeld – es geht um die Aspekte Lebensmitteltäuschung und Lebensmittelsicherheit. Eine effiziente und kostengünstige Untersuchungsmethode stellt das DNA‐basierte Next Generation Sequencing (NGS) dar ...
Sina Rammouz +5 more
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