Results 271 to 280 of about 40,506 (282)

An eDNA Survey of Plant Biodiversity in a Local Dam Within South Africa's Largest City. [PDF]

Ecol Evol
Nhlengethwa N, Stewart RD, Emami-Khoyi A, Teske PR, Csányi S, Heltai M, van der Bank M.   +6 more
europepmc   +1 more source

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Navigating the seven challenges of taxonomic reference databases in metabarcoding analyses

Molecular Ecology Resources, 2022
Assessment of biodiversity using metabarcoding data, such as from bulk or environmental DNA sampling, is becoming increasingly relevant in ecology, biodiversity sciences and monitoring.
F. Keck, Marjorie Couton, F. Altermatt
semanticscholar   +1 more source

Environmental DNA metabarcoding of freshwater fish in Malaysian tropical rivers using short-read nanopore sequencing as a potential biomonitoring tool.

Molecular Ecology Resources
The approach of combining cost-effective nanopore sequencing and emerging environmental DNA (eDNA) metabarcoding could prove to be a promising tool for biodiversity documentation, especially in Malaysia.
K. Munian, Farah Farhana Ramli, N. Othman, N. A. A. Mahyudin, N. H. Sariyati, Nurfatiha Akmal Fawwazah Abdullah-Fauzi, H. Haris, Mohd Lokman Ilham-Norhakim, M. A. Abdul-Latiff   +8 more
semanticscholar   +1 more source

Metabarcoding of Litter-Associated Fungi and Bacteria

2020
Litter-associated microorganisms play key roles in forested streams by decomposing and transferring energy from plant litter to higher trophic levels. To circumvent the shortcomings of traditional microbiological methods and conventional microscopy to assess the composition of microbial communities associated with decomposing litter, a range of DNA ...
Duarte, Sofia Alexandra Ferreira, Wurzbacher, Christian, Seena, Sahadevan   +2 more
openaire   +3 more sources

The future of eDNA metabarcoding

2018
Abstract Environmental DNA-based research is undergoing rapid developments, but its democratization in basic and applied research remains hampered by the biases introduced by molecular approaches, the difficulties in estimating absolute organisms’ abundances, and a lack of general consensus in molecular protocols.
Lucie Zinger, Eric Coissac, Pierre Taberlet, Aurélie Bonin   +3 more
openaire   +1 more source

Metabarcoding in gut protozoology

Trends in Parasitology
Next-generation sequencing (NGS) methods include whole-genome sequencing, metagenomic analysis, and amplicon-based NGS, all of which are gaining territory in parasitology. A modality of particular interest within the field of gut protozoology is exhaustive metabarcoding of ribosomal genes in a complex matrix such as faeces, by which method, amplicon ...
openaire   +2 more sources

DNA metabarcoding data analysis

2018
Abstract DNA metabarcoding generates huge amounts of data containing noise introduced by molecular methods. Chapter 8 “DNA metabarcoding data analysis” discusses the analytic steps and available software to curate and evaluate DNA metabarcoding data prior to final ecological analyses. It provides command lines to perform primary analyses
Eric Coissac, Pierre Taberlet, Lucie Zinger, Aurélie Bonin   +3 more
openaire   +1 more source

DNA metabarcode choice and design

2018
Abstract Chapter “DNA metabarcode choice and design” develops the properties of the ideal metabarcode in a given context, including conservation of the primer annealing regions and resolution power across the target taxonomic group of interest.
Pierre Taberlet, Eric Coissac, Lucie Zinger, Aurélie Bonin   +3 more
openaire   +1 more source

Using hierarchical models to compare the sensitivity of metabarcoding and qPCR for eDNA detection

Ecological Informatics, 2023
Emily F. McColl‐Gausden, A. Weeks, R. Coleman, S. Song, R. Tingley   +4 more
semanticscholar   +1 more source

obitools: a unix‐inspired software package for DNA metabarcoding

Molecular Ecology Resources, 2016
F. Boyer, C. Mercier, A. Bonin, Yvan Le Bras, P. Taberlet, É. Coissac   +5 more
semanticscholar   +1 more source