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Integrated multiplex PCR and metatranscriptomics reveal upper-lower airway microbial landscapes in pediatric respiratory infections. [PDF]

open access: yesVirol Sin
Wei P   +27 more
europepmc   +1 more source
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Application of metatranscriptomics to soil environments

Journal of Microbiological Methods, 2012
The activities of soil microbial communities are of critical importance to terrestrial ecosystem functioning. The mechanisms that determine the interactions between soil microorganisms, their environment and neighbouring organisms, however, are poorly understood.
Lilia C Carvalhais   +2 more
exaly   +6 more sources

Deciphering Metatranscriptomic Data

2014
Metatranscriptomic data contributes another piece of the puzzle to understanding the phylogenetic structure and function of a community of organisms. High-quality total RNA is a bountiful mixture of ribosomal, transfer, messenger and other noncoding RNAs, where each family of RNA is vital to answering questions concerning the hidden microbial world ...
Kopylova, Evguenia   +6 more
openaire   +4 more sources

Quantitative Microbial Metatranscriptomics

2014
The direct retrieval and sequencing of environmental RNA is emerging as a powerful technique to elucidate the in situ activities of microbial communities. Here we provide a metatranscriptomic protocol describing environmental sample collection, rRNA depletion, mRNA amplification, cDNA synthesis, and bioinformatic analysis.
Scott, Gifford   +2 more
openaire   +2 more sources

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