Advances and applications in sequencing-based pathogen surveillance. [PDF]
Luo H, Wang Y, Hou H, Yang J, Liu YX.
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Integrated multiplex PCR and metatranscriptomics reveal upper-lower airway microbial landscapes in pediatric respiratory infections. [PDF]
Wei P +27 more
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Integrated genomics, metagenomics and metatranscriptomics to reveal the biocontrol mechanism of <i>Bacillus velezensis</i> JY10 against tobacco target spot disease. [PDF]
Yang W +7 more
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Dynamics of the respiratory infectome in children with community-acquired pneumonia: insights from large and short time-scale analyses. [PDF]
Liao Y +7 more
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A Systematic Review on Microbial Profiling Techniques in Goat Milk: Implications for Probiotics and Shelf-Life. [PDF]
Monareng NJ +4 more
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Application of metatranscriptomics to soil environments
Journal of Microbiological Methods, 2012The activities of soil microbial communities are of critical importance to terrestrial ecosystem functioning. The mechanisms that determine the interactions between soil microorganisms, their environment and neighbouring organisms, however, are poorly understood.
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Deciphering Metatranscriptomic Data
2014Metatranscriptomic data contributes another piece of the puzzle to understanding the phylogenetic structure and function of a community of organisms. High-quality total RNA is a bountiful mixture of ribosomal, transfer, messenger and other noncoding RNAs, where each family of RNA is vital to answering questions concerning the hidden microbial world ...
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Quantitative Microbial Metatranscriptomics
2014The direct retrieval and sequencing of environmental RNA is emerging as a powerful technique to elucidate the in situ activities of microbial communities. Here we provide a metatranscriptomic protocol describing environmental sample collection, rRNA depletion, mRNA amplification, cDNA synthesis, and bioinformatic analysis.
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