Production of heterologous protein byMethylobacterium extorquensin high cell density fermentation [PDF]
The green fluorescent protein (GFP) was used as a model protein to study the recombinant protein production by the strain Methylobacterium extorquens ATCC 55366. Scale-up from shake flasks to 20 l fed-batch fermentation was achieved using methanol as a sole carbon and energy source and a completely minimal culture medium.
Bélanger, L. +7 more
openaire +3 more sources
Uptake and effect of rare earth elements on gene expression in Methylosinus trichosporium OB3b [PDF]
It is well known that Methylosinus trichosporium OB3b has two forms of methane monooxygenase (MMO) responsible for the initial conversion of methane to methanol, a cytoplasmic (soluble) methane monooxygenase and a membrane-associated (particulate ...
DiSpirito, Alan A +3 more
core +1 more source
Bacterial Species in Engineered Living Materials: Strategies and Future Directions
Bacterial ELMs can be derived from two sources: natural biofilms and hybrid living materials. In natural biofilms, the production of ELMs involves the genetic engineering of bacteria to manipulate the biofilm matrix components for desired material properties.
Hu Wang +3 more
wiley +1 more source
Characterization of Methylobacterium strains isolated from the phyllosphere and description of Methylobacterium longum sp. nov [PDF]
Methylobacterium strains are abundantly found in the phyllosphere of plants. Morphological, physiological and chemotaxonomical properties of 12 previously isolated strains were analyzed in order to obtain a more detailed overview of the characteristics ...
Bodelier, Paul +3 more
core
A novel protein for bioremediation of gadolinium waste
Abstract Several hundreds of tons of gadolinium‐based contrast agents (GBCAs) are being dumped into the environment every year. Although macrocyclic GBCAs exhibit superior stability compared to their linear counterparts, we have found that the structural integrity of chelates is susceptible to ultraviolet light, regardless of configuration.
Harvey D. Lee +10 more
wiley +1 more source
Media Selection for Poly(hydroxybutyrate) Production from Methanol by Methylobacterium Extorquens DSMZ 1340 [PDF]
Plackett-Burman design was used for selection of important media components such as carbon and nitrogen sources and minerals which affect poly(hydroxybutyrate) production and cell growth of Methylobacterium extorquens DSMZ 1340.
Zahra B. Mokhtari-Hosseini +4 more
doaj
Stress resistance and C1 metabolism involved in plant colonization of a methanotroph Methylosinus sp. B4S. [PDF]
Methanotrophs are widespread and have been isolated from various environments including the phyllosphere. In this study, we characterized the plant colonization by Methylosinus sp. B4S, an α-proteobacterial methanotroph isolated from plant leaf.
Iguchi, Hiroyuki +3 more
core +1 more source
Lanmodulin‐Decorated Microbes for Efficient Lanthanide Recovery
Bio‐scaffolded lanmodulin (LanM) on E. coli is developed as a simple, effective material for repeated capture and release of rare earth elements (REEs). Over 80% of four REE cations are captured, even in the presence of excess competitive ions. The material can be employed in a filtration flow‐through system with a capture capacity of 11.8 ± 0.9 mg g−1
Melanie Gut +6 more
wiley +1 more source
The PhyR homolog RSP_1274 of Rhodobacter sphaeroides is involved in defense of membrane stress and has a moderate effect on RpoE (RSP_1092) activity [PDF]
Background: A major role of the PhyR-NepR-sigma(EcfG) cascade in the general stress response was demonstrated for some bacterial species and considered as conserved in Alphaproteobacteria.
Klug, Gabriele, Li, Qingfeng, Peng, Tao
core +6 more sources
Evidence for corrin biosynthesis in the last universal common ancestor
Cobalamin biosynthesis can be traced back to the last universal common ancestor. After a stage of chemical evolution of the acetyl‐CoA pathway, the enzymatic synthesis of cobalamin evolved, allowing life to be independent of the insoluble solid‐state catalysts that tethered primordial CO2 assimilation to the Earth's crust, suggesting a role for corrin ...
Luca D. Modjewski +5 more
wiley +1 more source

