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Genetic modification of Methylobacterium extorquens G10 producer strain of polyhydroxybutyrate
Applied Biochemistry and Microbiology, 2014The effect of the increased copy number of polyhydroxybutyrate (PHB) biosynthesis genes in pink-pigmented methylobacterium Methylobacterium extorquens G10 on properties of the biopolymer was studied. The activity of poly-3-hydroxybutyril-synthase (PHB-synthase) was shown to increase and the molecular weight of synthesized PHB decreases twofold (150 -->
D N, Fedorov +4 more
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Nucleotide sequence of the amicyanin gene fromMethylobacterium extorquensAM1
DNA Sequence, 1991The gene for amicyanin from the methylotrophic bacterium, Methylobacterium extorquens AM1 was identified. It encodes a protein consisting of 119 amino acids with a molecular weight of 12,609 kDa. The amino acid sequence shows the presence of a typical leader sequence and signal peptidase recognition site. Two putative hairpin structures were found, one
A Y, Chistoserdov +2 more
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Journal of Molecular Biology, 1991
Single crystals of pseudoazurin, one of the blue copper proteins produced by methylotrophic bacterium Methylobacterium extorquens AM1, have been obtained by the method of vapor diffusion with ammonium sulfate as a precipitant at pH 8.0. Crystals belong to the orthorhombic system, space group P2(1)2(1)2(1), with unit cell dimensions of a = 52.619(4) A ...
T, Inoue +6 more
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Single crystals of pseudoazurin, one of the blue copper proteins produced by methylotrophic bacterium Methylobacterium extorquens AM1, have been obtained by the method of vapor diffusion with ammonium sulfate as a precipitant at pH 8.0. Crystals belong to the orthorhombic system, space group P2(1)2(1)2(1), with unit cell dimensions of a = 52.619(4) A ...
T, Inoue +6 more
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The Journal of Biochemistry, 1990
The electron transport system (with cytochrome aa3) coupled to the oxidation of methanol in Methylobacterium extorquens AM1 (former Pseudomonas AM1) was reconstituted with highly purified constituents of the system. A mixture of 2.7 microM methanol dehydrogenase, 3.2 microM cytochrome cH, and 71 nM cytochrome c oxidase (= cytochrome aa3) consumed ...
K, Mukai, Y, Fukumori, T, Yamanaka
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The electron transport system (with cytochrome aa3) coupled to the oxidation of methanol in Methylobacterium extorquens AM1 (former Pseudomonas AM1) was reconstituted with highly purified constituents of the system. A mixture of 2.7 microM methanol dehydrogenase, 3.2 microM cytochrome cH, and 71 nM cytochrome c oxidase (= cytochrome aa3) consumed ...
K, Mukai, Y, Fukumori, T, Yamanaka
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Термостабильность метанолдегидрогеназы, выделенной из Methylobacterium Extorquens PCM 160
VII Пущинская конференция «Биохимия, физиология и биосферная роль микроорганизмов», шко- ла-конференция для молодых ученых, аспирантов и студентов «Генетические технологии в микробио- логии и микробное разнообразие»С помощью метилотрофных бактерий Methylobacterium extorquens в настоящее время производят различные ценные химикаты, такие как, эктоин и полигидроксибутират, благодаря их способности использовать метанол в качестве единственного источника углерода и энергии (Chistoserdova L., 2003; Ю.А. Троценко, 2012).
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Клонирование и характеристика полигидроксибутиратсинтазы из Methylobacterium extorquens AM1
2016В результате поиска генов, кодирующих вероятные ПГБ-синтазы в геномах бактерий рода Methylobacterium, выявлены множественные (до пяти у одного штамма) гены ПГБ-синтаз. Филогенетическим анализом показано, что белки PhaC1, PhaC2, PhaC3 относятся к I классу ПГБ-синтаз, белки PhaC4 – к ПГБ-синтазам III класса, тогда как PhaC5, по-видимому, представляет ...
Zamakhaeva, Svetlana A. +3 more
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Physiological analysis of Methylobacterium extorquens AM1 grown in continuous and batch cultures
Archives of Microbiology, 2006Chemostat cultures of Methylobacterium extorquens AM1 grown on methanol or succinate at a range of dilution rates were compared to batch cultures in terms of enzyme levels, poly-beta-hydroxybutyrate content, and intracellular concentrations of adenine and pyridine nucleotides. In both chemostat and batch cultures, enzymes specific to C1 metabolism were
Xiaofeng, Guo, Mary E, Lidstrom
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2010
The paper deals with action efficiency of microbial biomass on characteristic indicators at alcohol and morphine organism intoxication. The investigated microbial biomass affects the regulatory biochemical and physiological systems in experimental animals, normalizes activity of alcohol dehydrogenase and aldehide dehydrogenase, as well as the content ...
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The paper deals with action efficiency of microbial biomass on characteristic indicators at alcohol and morphine organism intoxication. The investigated microbial biomass affects the regulatory biochemical and physiological systems in experimental animals, normalizes activity of alcohol dehydrogenase and aldehide dehydrogenase, as well as the content ...
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The genetic analysis of the facultative methylotroph, methylobacterium extorquens AM1
2021This thesis was scanned from the print manuscript for digital preservation and is copyright the author. Researchers can access this thesis by asking their local university, institution or public library to make a request on their behalf. Monash staff and postgraduate students can use the link in the References field.
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SEVERE PNEUMONIA WITH SEPSIS DUE TO METHYLOBACTERIUM EXTORQUENS.
Southern Medical Journal, 1992Yogesh Patel, Charles Gutierrez.
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