Results 71 to 80 of about 1,121 (154)
Induction of autophagy impairs the survival of Mfa1+Pg strain within MoDCs.
A) Survival of P. gingivalis strains within MoDCs after 6, 12, 24 and 48 hours. Blue lines show P. gingivalis strains survival within MoDCs and their survival in anaerobic condition in the absence of DCs are showed in black lines. The effect of rapamycin
Jennifer L. Waller (357473) +11 more
core +1 more source
Polyamine homeostasis is coupled with the cAMP–PKA pathway to control the dimorphic switch of sugarcane smut fungus by modulating the intracellular reactive oxygen species levels. The image was created by Biorender. Abstract Sugarcane smut caused by Sporisorium scitamineum seriously impairs sugarcane production and quality.
Kai Yin +5 more
wiley +1 more source
Blocking of DC-SIGN inhibits Mfa1+Pg uptake and restores basal LC3-II signal in MoDCs.
A) Immuno-fluorescence images of LC3-II and P. gingivalis within MoDCs pre-treated with GP120 (DC-SIGN blocker) after 12 hours of infection. LC3-II was detected in red-fluorescent dye and the bacterial strains were pre-labeled with green CFSE.
Jennifer L. Waller (357473) +11 more
core +1 more source
The “α-op×α-op” cross: 9.6×107 white cells of “helper” strains (60%) were mixed with 3.2×107 opaque cells of SZ306α (Δ/α, ura3Δ/Δ, 20%) and 3.2×107 opaque cells of GH1349 (α/α, arg4Δ/Δ, 20%). The “a-op(mfa1Δ/Δ)×α-op” cross: 9.6×107 white cells of “helper”
Qiuyu Zhang (545562) +6 more
core +1 more source
Low LC3-II signals in human MoDCs infected with Mfa1+Pg.
A) Epifluorescence microscopy images of MoDCs infected with Pg381, Mfa1+Pg, FimA+Pg and MFB strains after 12 hours. LC3-II was detected in red-fluorescent (Texas red) dye and the bacterial strains were pre-labeled with CFSE (green). Co-localization of P.
Jennifer L. Waller (357473) +11 more
core +1 more source
Isolation and survey of novel fluoroacetate-degrading bacteria belonging to the phylum Synergistetes
Synergistetes strain MFA1 is an asaccharolytic ruminal bacterium isolated based on its ability to degrade fluoroacetate, a plant toxin. The amino acid and peptide requirements of the bacterium were investigated under different culturing conditions.
Davis, Carl K. +4 more
core +1 more source
Electrophoretic analyses of PGN_1808 polymerization.
Fractionated products of PGN_1808 (1808), FimA and Mfa1 in SDS-containing buffer were heated at 37 (1), 60 (2), 80 (3) and 100 (4)°C for 10 min, and then applied to SDS-PAGE.
Yoshiaki Hasegawa (136300) +3 more
core +1 more source
Formation of double-membrane vesicles in P. gingivalis-infected MoDCs.
A) Scanning electron microscopy (SEM) of MoDCs of the early interaction of MoDCs with Pg381 and Mfa1+Pg (upper panel 10000x and lower panel 25000x). B) SEM for MoDCs interacting with Pg381 (green stains for bacteria are computer generated).
Jennifer L. Waller (357473) +11 more
core +1 more source
Immunoblot analysis for PgmA using whole-cell sonicates.
Whole-cell sonicates (W) were fractionated into soluble (Sol), envelope (Env), inner membrane (IM) and outer membrane (OM) fractions. Samples were denatured in an SDS-containing buffer with 2-mercaptoethanol by heating at 100°C for 10 min, then subjected
Yoshiaki Hasegawa (136300) +5 more
core +1 more source
Mfa5 expression and localization in P. gingivalis JI-1, FMFA4, and FMFA4C.
Culture supernatants (CS) and whole-cell lysates (W) from JI-1, FMFA4, and FMFA4C were separated by SDS-PAGE and probed with Mfa5 antibodies by immunoblotting.
Yoshiaki Hasegawa (136300) +8 more
core +1 more source

