Results 131 to 140 of about 165,529 (277)

Evaluation of Spontaneous Pain in a Monosodium Iodoacetate-Induced Mouse Model of Osteoarthritis Using the Mouse Grimace Scale. [PDF]

Biomedicines
Alnoud M, Rios J, Szobody MW, Usman AA, Rodriguez A, Nguyen TA, Ayala S, Quraishi AS, Salinas M, Benamar K.   +9 more
europepmc   +1 more source

Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection

FEBS Open Bio, EarlyView.
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow, Isabel Brandtjen, Yaxin Mo, Melissa Gulley, Louis Osorio, Sagar Kudchodkar, Nandita C. Jhumur, Christine C. Roberts, Lisa K. Denzin, David I. Shreiber, Biju Parekkadan, Hao Lin, Joel N. Maslow   +12 more
wiley   +1 more source

Artificial intelligence-based automated scoring of the mouse grimace scale: a systematic review. [PDF]

Pain Rep
Rabierad M, Schulz L, Kumstel S, von Lukas UF, Vollmar B, Zechner D.   +5 more
europepmc   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Genetic predictive links between oropharyngeal microecology and obstructive sleep apnea syndrome: expanding the perspective of etiology. [PDF]

J Oral Microbiol
Zhao L, Zhang Y, Lin L, Wang T, Ding Y, Wang W, Zhao Z, Han J, Liu L, Zeng Y.   +9 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Interactions of Mucomimetic Polymers and Meibomian Surface Films upon Exposure to Environmental Stressors. [PDF]

Biomolecules
Georgiev GA, Yokoi N, Kim F, Bacheva M, Nishiyama M, Hotta T.   +5 more
europepmc   +1 more source

De novo synthesis of short‐chain aldehydes and hydrocarbons secreted by the brown marmorated stink bug Halyomorpha halys

FEBS Open Bio, EarlyView.
The brown marmorated stink bug, Halyomorpha halys, mainly secretes 4‐oxo‐(E)‐2‐hexenal, (E)‐2‐decenal, dodecane and tridecane, a mixture representative of that released by many heteropterans. We demonstrated that H. halys can synthesise these secretory components de novo using dietary glucose.
Haruna Fujimori, Koji Noge
wiley   +1 more source

A prospective study of familial predisposition to plasma cell dyscrasias. [PDF]

Blood Adv
Gavriatopoulou M, Andrikopoulou A, Ntanasis-Stathopoulos I, Migkou M, Kanellias N, Eleutherakis-Papaiakovou E, Kokkali NA, Malandrakis P, Liacos CI, Bagratuni T, Theodorakakou F, Filippatos C, Fotiou D, Terpos E, Kastritis E, Dimopoulos MA.   +15 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source