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Quartz micropipettes for intracellular voltage microelectrodes and ion-selective microelectrodes
Journal of Neuroscience Methods, 1987We find that quartz is better than borosilicate glass as a material for micropipettes for certain microelectrode applications, including ion-selective microelectrodes of the liquid membrane type. Details are given of a graphite heating element that can be mounted on a standard microelectrode puller and used for making quartz micropipettes.
Munoz JL, Coles JA
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1996
A review with 71 refs. discussing amperometric and potentiometric microelectrodes and their principles of operation, as well as some practical applications of microelectrodes, such as in studies of mass boundary layers, detn. of effective diffusion coeffs. in biofilms, and studies of activity distribution in methanogenic aggregates.
Ottengraf, S.P.P., van den Heuvel, J.C.
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A review with 71 refs. discussing amperometric and potentiometric microelectrodes and their principles of operation, as well as some practical applications of microelectrodes, such as in studies of mass boundary layers, detn. of effective diffusion coeffs. in biofilms, and studies of activity distribution in methanogenic aggregates.
Ottengraf, S.P.P., van den Heuvel, J.C.
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Microelectrodes in microbial ecology
Analytical Chemistry, 1989Understanding the microenvironment of bacteria has presented many challenges for the microbial ecologist. Simple intracellular capillary electrodes have been used in neurophysiology since the 1950s to measure action potentials in ion transport over biological membranes, and ion-selective electrodes were developed soon thereafter for the determination ...
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Another tungsten microelectrode
Medical & Biological Engineering, 1972A technique is described for fitting a sharpened tungsten wire and a glass micropipette together so that a precisely controlled amount of bare wire is exposed at the tip. The method is tedious and demanding but produces very reliable electrodes yielding isolated extracellular recordings from single nerve cells and their axons.
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Ca2+-selective microelectrodes
Cell Calcium, 1985Ca2+-selective microelectrodes based on the synthetic neutral carrier ETH 1001 can be used for quantitative intracellular measurements of resting Ca2+-activities and of slowly changing Ca2+-levels (response time in the order of seconds). Microelectrodes with tip diameters greater than 0.3 micron show selectivities that yield a detection limit between ...
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Intracellular Oxygen Microelectrodes
1973In this presentation the work of many associates of mine will be discussed, most notably Dr. Pankajam Nair. I will say little about the construction of the micro O2 electrode since the method is essentially the same as has been published (7). Rather, I will first briefly discuss tests of its reliability and new calibration techniques; then some of our ...
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1974
The important requirements of an intracellular pH microelectrode are: A. A sharp tip to minimize cell wall damage on penetration. C. A smooth transition between the pH sensing portion and the insulated portion. D. A conical angle as small as possible for the portion of the microelectrode that will be in the cell wall.
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The important requirements of an intracellular pH microelectrode are: A. A sharp tip to minimize cell wall damage on penetration. C. A smooth transition between the pH sensing portion and the insulated portion. D. A conical angle as small as possible for the portion of the microelectrode that will be in the cell wall.
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