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Microinjection of RNA into Mouse Zygotes
Cold Spring Harbor Protocols, 2018This protocol describes the injection of RNA into zygotes.
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Chapter 25 Microinjection of Tetrahymena thermophila
1999Publisher Summary Microinjection has proved to be a powerful experimental tool used to introduce macromolecules into cells. The method is widely used for DNA-mediated transformation of diverse organisms including ciliates, nematodes, insects, and mammals.
Douglas L. Chalker +3 more
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Microinjection and transgenesis
Biochimie, 1998Alejandro García-Carrancá +1 more
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Isolation of DNA Fragments for Microinjection
2003The purification of a DNA fragment for microinjection is extremely important. This chapter describes a rapid and efficient technique for isolating specific DNA fragments from agarose gels run in Tris-acetate buffer, and was first described by Vogelstein and Gillespie.
Smith, D.R., Murphy, D.
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Quantitative Microinjection of Mosquitoes
Science, 1956Gordon H. Ball, Jowett Chao
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Microinjection of mosquito embryos
1997Due to the recent interest in the development of novel genetic methods to control insect vectors of disease, a great deal of research has focused on the genetic manipulation of the insect vector (James, 1992). In the design of a control programme involving genetic modification of the insect vector, factors to be considered include the isolation of ...
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Single-Cell Microinjection Technologies
2012Single-cell microinjection, as a mechanical delivery tool, has been used for transferring substances into transfection or infection challenging cells. Here, we discuss the advantages and applications of microinjection, list the materials needed for performing microinjection experiments, and describe the methods of single-cell microinjection into ...
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