Results 101 to 110 of about 21,579 (256)
Generating golden Syrian hamsters with conditional alleles via zygote microinjection of CRISPR/Cas9
Animal Models and Experimental Medicine, EarlyView.We established the first conditional knockout (cKO) model in the golden Syrian hamster by CRISPR/Cas9‐mediated genome editing. Cas9 protein, two sgRNAs, and a donor plasmid carrying loxP‐flanked exon 2 of the ApoF gene were microinjected into one‐cell embryos. The floxed allele was efficiently generated (up to 27%) and transmitted through the germline.
Wei Chen +16 more
wiley +1 more source
Recursive Least Squares Filtering Algorithms for On-Line Viscoelastic Characterization of Biosamples
Actuators, 2018 The mechanical characterization of biological samples is a fundamental issue in biology and related fields, such as tissue and cell mechanics, regenerative medicine and diagnosis of diseases.
Paolo Di Giamberardino +3 more
doaj +1 more source
Micromachines, 2017 Dielectrophoresis is defined as the motion of an electrically polarisable particle in a non-uniform electric field. Current dielectrophoretic devices enabling sorting of cells are mostly controlled in open-loop applying a predefined voltage on micro ...
Vladimir Gauthier +2 more
doaj +1 more source
SmartBot, EarlyView.Ionogels dynamically and functionally couple ionic liquids with polymer networks, enabling multiscale programmable deformation and motion under electrical, magnetic, optical, thermal, humidity, and chemical stimuli. This article systematically elucidates the constituent components, structural design, and multiscale actuation mechanisms of ionogel ...
Dezhou Cao +5 more
wiley +1 more source
Robotic Cell Micromanipulation Skill Learning via Imitation‐Enhanced Reinforcement Learning
CAAI Transactions on Intelligence Technology, EarlyView.ABSTRACT Humans can learn complex and dexterous manipulation tasks by observing videos, imitating and exploring. Multiple end‐effectors manipulation of free micron‐sized deformable cells is one of the challenging tasks in robotic micromanipulation. We propose an imitation‐enhanced reinforcement learning method inspired by the human learning process ...
Youchao Zhang +6 more
wiley +1 more source
Immune involvement in neuropsychiatric disorders: Insights from single‐cell transcriptomic studies
Psychiatry and Clinical Neurosciences, EarlyView.Neuropsychiatric disorders pose profound challenges to both research and treatment, largely due to their clinical heterogeneity and the limited understanding of their underlying biological mechanisms. While bulk RNA sequencing (bulk RNA‐seq) has been widely used to study gene expression, it cannot resolve cell‐type‐specific signals or detect rare ...
Tsutomu Takeda +4 more
wiley +1 more source
Advanced trajectory engineering of diffraction-resisting laser beams [PDF]
, 2013 We introduce an analytical technique for engineering the trajectory of diffraction-resisting laser beams. The generated beams have a Bessel-like transverse field distribution and can be navigated along rather arbitrary curved paths in free space, thus ...
Chen, Zhigang +3 more
core
Studies on the non-specific esterases of Saccharomyces cerevisiae : a thesis in partial fulfillment [sic.] of the requirements for the degree of Master of Science in Microbiology at Massey University [PDF]
, 1984 Twenty wine-making and three laboratory strains of Saccharomyces cerevisiae were examined for non-specific esterases by Polyacrylamide Gel Electrophoresis.
Dick, Bruce
core
, 2008 Two-photon photopolymerization of UV curing resins is an attractive method for the fabrication of microscopic transparent objects with size in the tens of micrometers range.
Deitz D +8 more
core +1 more source
Potential of the acoustic micromanipulation technologies for biomedical research [PDF]
, 2021 Fatih Akkoyun +2 more
openalex +1 more source