Results 31 to 40 of about 198,505 (311)

Specific antigen/antibody interactions measured by force microscopy

, 1996
Dammer U, Hegner M, Anselmetti D, et al. Specific antigen/antibody interactions measured by force microscopy. Biophysical journal. 1996;70(5):2437-2441.Molecular recognition between biotinylated bovine serum albumin and polyclonal, biotin-directed IgG ...
Dreier, M., Dammer, Ulrich, Hegner, M., H.J. Güntherodt, Güntherodt, H.J., Wagner, Peter, U. Dammer, M. Dreier, Wagner, P., D. Anselmetti, Hegner, Martin, Huber, Walter, Güntherodt, Hans-Joachim, Huber, W., W. Huber, Anselmetti, D., Dreier, Markus, Dammer, U., P. Wagner, Anselmetti, Dario ; https://orcid.org/, M. Hegner   +20 more
core   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Microrheology of human lung epithelial cells measured by atomic force microscopy

, 2003
Lung epithelial cells are subjected to large cyclic forces from breathing. However, their response to dynamic stresses is poorly defined. We measured the complex shear modulus (G*(ω)) of human alveolar (A549) and bronchial (BEAS-2B) epithelial cells over
Farré, Ramon, Buscemi Estefanell, Lara, Trepat Guixer, Xavier, Alcaraz, Jordi, Farré Ventura, Ramon, Buscemi, Lara, Trepat, Xavier, Grabulosa Descals, Mireia, Alcaraz Casademunt, Jordi, Grabulosa, Mireia, Navajas, Daniel, Fabry, Ben, Navajas Navarro, Daniel   +12 more
core   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Friction force microscopy : a simple technique for identifying graphene on rough substrates and mapping the orientation of graphene grains on copper [PDF]

, 2013
At a single atom thick, it is challenging to distinguish graphene from its substrate using conventional techniques. In this paper we show that friction force microscopy (FFM) is a simple and quick technique for identifying graphene on a range of samples,
Wilson, Neil R., Marsden, Alexander J., Phillips, Mick   +2 more
core   +1 more source

Organizing the interface—Plasma membrane architecture and receptor dynamics in virus‐cell interactions

FEBS Letters, EarlyView.
Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley   +1 more source

Pectin Nanostructure Visualization by Atomic Force Microscopy

Bio-Protocol, 2015
Pectins, complex polysaccharides rich in galacturonic acid, are a major component of primary plant cell walls. These macromolecules regulate cell wall porosity and intercellular adhesion, being important in the control of cell expansion and ...
Sara Posé, Candelas Paniagua, Andrew Kirby, A. Gunning, Victor Morris, Miguel Quesada, Jose Mercado   +6 more
doaj   +1 more source

Biophysical approaches for studying viral entry

FEBS Letters, EarlyView.
Viruses infect all living organisms and have been responsible for major epidemics and pandemics. Their ongoing evolutionary battle with host defenses creates a constant need for improved tools to study viral behavior. Advancing methods to probe viral attachment, fusion, and genome release deepen our understanding of how infections begin and support the
Inbar Yosibash, Raya Sorkin
wiley   +1 more source

SNAREs: Could They be the Answer to an Energy Landscape Riddle in Exocytosis?

The Scientific World Journal, 2010
During exocytosis, chemical transmitters stored in secretory vesicles can be released upon fusion of these intracellular organelles to the plasma membrane. In this process, SNARE proteins that form a ternary core complex play a central role. This complex
Wei Liu, Vladimir Parpura
doaj   +1 more source

High-precision atomic force microscopy with atomically-characterized tips

New Journal of Physics, 2020
Traditionally, atomic force microscopy (AFM) experiments are conducted at tip–sample distances where the tip strongly interacts with the surface. This increases the signal-to-noise ratio, but poses the problem of relaxations in both tip and sample that ...
A Liebig, A Peronio, D Meuer, A J Weymouth, F J Giessibl   +4 more
doaj   +1 more source