Results 31 to 40 of about 381,285 (254)

Methanol fixation for scanning electron microscopy of plants

Applied Microscopy, 2020
Plant specimens for scanning electron microscopy (SEM) are commonly treated using standard protocols. Conventional fixatives consist of toxic chemicals such as glutaraldehyde, paraformaldehyde, and osmium tetroxide.
Ki Woo Kim
doaj   +1 more source

Cryoprotection of porcine cornea: a scanning electron microscopy study

Veterinární Medicína, 2005
Porcine corneas were frozen with Me2SO, glycerol, 1,2-propanediol and PEG-400. The effects of the range of concentrations (5% and 10%) and temperature regimen (1ºC/min and 5ºC/min) were investigated.
A. Neronov, P. Giurov, M. Cholakova, M. Dimitrova, E. Nikolova   +4 more
doaj   +1 more source

Electron counting detectors in scanning transmission electron microscopy via hardware signal processing

Nature Communications, 2023
Transmission electron microscopy is a pivotal instrument in materials and biological sciences due to its ability to provide local structural and spectroscopic information on a wide range of materials.
Jonathan J. P. Peters, Tiarnan Mullarkey, Emma Hedley, Karin H. Müller, Alexandra Porter, Ali Mostaed, Lewys Jones   +6 more
doaj   +1 more source

Ultrastructural aspects of pseudoxanthoma elasticum [PDF]

Anais Brasileiros de Dermatologia, 2017
: We report the ultrastructural findings in a case of a 72-year-old black woman with confluent yellowish papules in the cervical region. She had no comorbidities.
Hiram Larangeira de Almeida Jr., Mariana Geber de Almeida, Valéria Magalhães Jorge, Luciana Boff de Abreu   +3 more
doaj   +1 more source

Scanning Electron Microscopy

Current Protocols
AbstractScanning electron microscopy (SEM) remains distinct in its ability to allow topographical visualization of structures. Key elements to consider for successful examination of biological specimens include appropriate preparative and imaging techniques.
Elizabeth R. Fischer, Bryan T. Hansen, Vinod Nair, Forrest H. Hoyt, Cindi L. Schwartz, David W. Dorward   +5 more
openaire   +2 more sources

Neutrophil deficiency increases T cell numbers at the site of tissue injury in mice

FEBS Letters, EarlyView.
In wild‐type mice, injury or acute inflammation induces neutrophil influx followed by macrophage accumulation. Mcl1ΔMyelo (neutrophil‐deficient) mice lack neutrophils, and in response to muscle injury show fewer macrophages and exhibit strikingly elevated T‐cell numbers, primarily non‐conventional “double‐negative” (DN) αβ and γδ T cells.
Hajnalka Halász, Albert Bálint Papp, Lukács Sándor Lesinszki, Attila Mócsai, Tamás Varga, László Nagy, Péter Gogolák   +6 more
wiley   +1 more source

Comprehensive profiling of lncRNAs and mRNAs enriched in small extracellular vesicles for early noninvasive detection of colorectal cancer: diagnostic panel assembly and extensive validation

Molecular Oncology, EarlyView.
Small extracellular vesicles are a promising source of diagnostic molecules. We conducted a comprehensive study, including transcriptome profiling and RT‐qPCR validation on large cohorts of samples. Diagnostic panels enabling sensitive detection of colorectal cancer and precancerous lesions were established. Some molecules were differentially expressed
Petra Vychytilova‐Faltejskova, Marketa Pavlikova, Lucie Pifkova, Robin Jugas, Tana Machackova, Lenka Radova, Milana Sachlova, Renata Bartosova, Tetiana Samoilenko, Zuzana Feckova, Jana Orlickova, Erika Slamova, Elleni Ponechal Michu, Dagmar Al Tukmachi, Michaela Ruckova, Martina Vodinska, Jan Kotoucek, Tina Catela Ivkovic, Marie Boudna, Lucia Bohovicova, Teodor Stanek, Jana Halamkova, Martin Svoboda, Vladimir Prochazka, Tomas Grolich, Zdenek Kala, Ondrej Slaby   +26 more
wiley   +1 more source

Analysis and applications of a heralded electron source

New Journal of Physics
We analytically describe the noise properties of a heralded electron source made from a standard electron gun, a weak photonic coupler, a single photon counter, and an electron energy filter.
Stewart A Koppell, John W Simonaitis, Maurice A R Krielaart, William P Putnam, Karl K Berggren, Phillip D Keathley   +5 more
doaj   +1 more source

The impact of frailty syndrome on skeletal muscle histology: preventive effects of exercise

FEBS Open Bio, EarlyView.
Frailty syndrome exacerbates skeletal muscle degeneration via increased ECM deposition and myofiber loss. This study, using a murine model, demonstrates that endurance exercise attenuates these histopathological alterations, preserving muscle integrity. Findings support exercise as a viable strategy to counteract frailty‐induced musculoskeletal decline
Fujue Ji, Hae Sung Lee, Haesung Lee, Jong‐Hee Kim   +3 more
wiley   +1 more source

Biophysical characterization and ion transport with cell‐based and proteoliposome reconstitution assays of invertebrate K+‐Cl− cotransporters

FEBS Open Bio, EarlyView.
The K+‐Cl− cotransporters (KCCs) facilitate the symport of ions across the plasma membrane. They participate in physiological processes including neuronal regulation. Here, we characterized KCCs from Drosophila and Hydra vulgaris. Comparative analyses of transporters provide insights into the mechanism of KCC ion transport, regulation, and evolution ...
Satoshi Fudo, Marina Verkhovskaya, Coralie Di Scala, Claudio Rivera, Tommi Kajander   +4 more
wiley   +1 more source