Electron microscopic measurement of the size of the optical focus in laser scanning microscopy [PDF]
We describe a method for measuring the lateral focal spot size of a multiphoton laser scanning microscope (LSM) with unprecedented accuracy. A specimen consisting of an aluminum film deposited on a glass coverslip was brought into focus in a LSM and the ...
McConnell, Gail
core +4 more sources
Phosphogypsum surface characterisation using scanning electron microscopy [PDF]
This paper presents the results of application of Scanning Electron Microscopy (SEM) to examinations of the samples of natural gypsum and phosphogypsum.
Rajković Miloš B. +1 more
doaj +1 more source
SCANNING ELECTRON MICROSCOPY [PDF]
Abstract Scanning electron microscopy is a method of microscopy that permits resolution better than that of the optical microscope (about 100 A) while examining one surface of a bulk specimen. The technique depends on electronic application to microscopy and has been developed over many years by electronic engineers rather than physicists or ...
openaire +2 more sources
Morphologic observations of human T and B lymphocytes by scanning electron microscopy [PDF]
T cell concerned with cell mediated immunity and B cell concerned with humoral antibody are classified by scanning electron microscopy of the surface structure of lymphocytes using E binding lymphocytes and EAC (sensitized sheep erythrocytes treated with
Yamamoto, Isamu
core +1 more source
Chromosome Centromeres: Structural and Analytical Investigations with High Resolution Scanning Electron Microscopy in Combination with Focused Ion Beam Milling [PDF]
Whole mount mitotic metaphase chromosomes of different plants and animals were investigated with high resolution field emission scanning electron microscopy (FESEM) to study the ultrastructural organization of centromeres, including metacentric ...
Schroeder-Reiter, E., Wanner, Gerhard
core +1 more source
AbstractScanning electron microscopy (SEM) remains distinct in its ability to allow topographical visualization of structures. Key elements to consider for successful examination of biological specimens include appropriate preparative and imaging techniques.
Elizabeth R. Fischer +5 more
openaire +2 more sources
Scanning ultrafast electron microscopy [PDF]
Progress has been made in the development of four-dimensional ultrafast electron microscopy, which enables space-time imaging of structural dynamics in the condensed phase. In ultrafast electron microscopy, the electrons are accelerated, typically to 200 keV, and the microscope operates in the transmission mode.
Yang, Ding-Shyue +2 more
openaire +3 more sources
Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley +1 more source
Quantitative Local Photosynthetic Flux Measurements at Isolated Chloroplasts and Thylakoid Membranes Using Scanning Electrochemical Microscopy (SECM) [PDF]
Scanning electrochemical microscopy (SECM) offers a fast and quantitative method to measure local fluxes within photosynthesis. In particular, we have measured the flux of oxygen and ferrocyanide (Fe(CN)64–), from the artificial electron acceptor ...
Robinson, Colin +9 more
core +1 more source
Encapsulins are protein nanocompartments that play an important role in iron storage. In the Myxococcus xanthus encapsulin system, two cargo proteins called EncB and EncC contribute to iron mineralization. Here, we show that EncB and EncC generate iron‐containing minerals with distinct chemical compositions, suggesting that the composition of stored ...
Harry B. McDowell +2 more
wiley +1 more source

