Results 241 to 250 of about 625,799 (289)
Some of the next articles are maybe not open access.
Current Protocols in Immunology, 1998
AbstractAbsorption of energy as light by some molecules and emission of energy as fluorescence can occur only at certain wavelengths, which are characteristic for a given molecule (fluorophore). Fluorescence microscopes are equipped to observe the fluorescence of one or more specific fluorophores to localize specific molecules and analyzed cellular ...
openaire +5 more sources
AbstractAbsorption of energy as light by some molecules and emission of energy as fluorescence can occur only at certain wavelengths, which are characteristic for a given molecule (fluorophore). Fluorescence microscopes are equipped to observe the fluorescence of one or more specific fluorophores to localize specific molecules and analyzed cellular ...
openaire +5 more sources
Fluorescent Speckle Microscopy
Cold Spring Harbor Protocols, 2011INTRODUCTIONFluorescent speckle microscopy (FSM) is a live imaging and quantitative measurement technique used for analyzing motion and turnover of macromolecular assemblies in vivo and in vitro. It differs from related imaging techniques such as photobleaching and photoactivation in its use of substantially lower concentrations of fluorescently ...
Benjamin R. Houghtaling +2 more
openaire +2 more sources
Fundamentals of Fluorescence and Fluorescence Microscopy
2003This chapter discusses the fundamental physics of fluorescence. The application of fluorescence to microscopy represents an important transition in the development of microscopy, particularly as it applies to biology. It enables quantitating the amounts of specific molecules within a cell, determining whether molecules are complexing on a molecular ...
openaire +5 more sources
Factors in Fluorescence Microscopy
Journal of the Optical Society of America, 1947This paper is concerned with a presentation and an attempted evaluation of the various factors governing successful observations with the microscope using fluorescence technics. The primary considerations are: (1) brightness of the fluorescent image in absolute terms and (2) brightness level, image contrast and resolving power of the eye.
Charles Sheard, D. H. Hamly
openaire +3 more sources
Digital Fluorescence Microscopy
Current Protocols in Cytometry, 1997AbstractFluorescence microscopy is a valuable tool for biomedical research, providing good sensitivity and high multiplicity. Specialized techniques (anisotropy measurement, resonance energy transfer) can provide information on cell spatial arrangement or label microenvironment.
openaire +3 more sources
Nature Methods, 2005
Fluorescence microscopy has undergone a renaissance in the last decade. The introduction of green fluorescent protein (GFP) and two-photon microscopy has allowed systematic imaging studies of protein localization in living cells and of the structure and function of living tissues.
openaire +3 more sources
Fluorescence microscopy has undergone a renaissance in the last decade. The introduction of green fluorescent protein (GFP) and two-photon microscopy has allowed systematic imaging studies of protein localization in living cells and of the structure and function of living tissues.
openaire +3 more sources
Fluorescence (Multiwave) Confocal Microscopy
Dermatologic Clinics, 2016In addition to reflectance confocal microscopy, multiwave confocal microscopes with different laser wavelengths in combination with exogenous fluorophores allow fluorescence mode confocal microscopy in vivo and ex vivo. Fluorescence mode confocal microscopy improves the contrast between the epithelium and the surrounding soft tissue and allows the ...
Welzel, Julia +2 more
openaire +4 more sources
1980
Publisher Summary Fluorescence microscopy is based on the principle that light of short wavelength, such as ultraviolet light, will excite certain dyes known as fluorochromes to emit visible light of longer wavelength that can be observed as fluorescence, when the correct type of filter is placed between the objective and eyepiece of the microscope ...
P.S. GARDNER, J. McQUILLIN
openaire +2 more sources
Publisher Summary Fluorescence microscopy is based on the principle that light of short wavelength, such as ultraviolet light, will excite certain dyes known as fluorochromes to emit visible light of longer wavelength that can be observed as fluorescence, when the correct type of filter is placed between the objective and eyepiece of the microscope ...
P.S. GARDNER, J. McQUILLIN
openaire +2 more sources
Introduction to Fluorescence Microscopy
2011This chapter is an overview of basic principles of fluorescence microscopy, including a brief history on the invention of this type of microscopy. The chapter highlights important points related to properties of fluorochromes, resolution in fluorescence microscopy, phase contrast and fluorescence, fluorescence filters, construction of a fluorescence ...
openaire +3 more sources
Fluorescence Microscopy and Fluorescent Probes
1996Invited Review Lectures: Recent Developments in Fluorescence Microscopy Long-Lived Metal-Ligand Probes Three-Photon Excitation and Optical Control of Excited State Population J.R. Lakowicz, et al. Computer Simulations of the Behavior of Hydrophobic Probe Molecules in Lipid Bilayers H.C. Gerritsen, Y.K. Levine.
Fluorescent Probes, Jan Slavík
openaire +2 more sources