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2019
In this two-part chapter, the background to confocal microscopy and two-photon fluorescence microscopy is first presented, with a detailed description of the optical setup. This is followed by a critical account of the many super-resolution techniques: coordinated stochastic fluorescence microscopy (photoactivation localization microscopy ...
Alberto Diaspro +7 more
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In this two-part chapter, the background to confocal microscopy and two-photon fluorescence microscopy is first presented, with a detailed description of the optical setup. This is followed by a critical account of the many super-resolution techniques: coordinated stochastic fluorescence microscopy (photoactivation localization microscopy ...
Alberto Diaspro +7 more
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Fluorescence (Multiwave) Confocal Microscopy
Dermatologic Clinics, 2016In addition to reflectance confocal microscopy, multiwave confocal microscopes with different laser wavelengths in combination with exogenous fluorophores allow fluorescence mode confocal microscopy in vivo and ex vivo. Fluorescence mode confocal microscopy improves the contrast between the epithelium and the surrounding soft tissue and allows the ...
Welzel, Julia +2 more
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Nature Methods, 2005
Fluorescence microscopy has undergone a renaissance in the last decade. The introduction of green fluorescent protein (GFP) and two-photon microscopy has allowed systematic imaging studies of protein localization in living cells and of the structure and function of living tissues.
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Fluorescence microscopy has undergone a renaissance in the last decade. The introduction of green fluorescent protein (GFP) and two-photon microscopy has allowed systematic imaging studies of protein localization in living cells and of the structure and function of living tissues.
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Digital Fluorescence Microscopy
Current Protocols in Cytometry, 1997AbstractFluorescence microscopy is a valuable tool for biomedical research, providing good sensitivity and high multiplicity. Specialized techniques (anisotropy measurement, resonance energy transfer) can provide information on cell spatial arrangement or label microenvironment.
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Digital imaging fluorescence microscopy
1986For digital imaging fluorescence microscopy we have used a Grinnell 274 image processor interfaced to a Lab Datex LSI 11/23 under RT-11 controlled by Integrated Solutions 68010 operating under Unix BSD 4.2. Experimental approaches to address (a) photobleaching of the fluorophore, (b) misalignment of images by nonparfocal microscope optics and (c) the ...
Smith, L.C. +3 more
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Quantitative Fluorescence Microscopy Techniques
2009Fluorescence microscopy is a non-invasive technique that allows high resolution imaging of cytoskeletal structures. Advances in the field of fluorescent labelling (e.g., fluorescent proteins, quantum dots, tetracystein domains) and optics (e.g., super-resolution techniques and quantitative methods) not only provide better images of the cytoskeleton ...
ESPOSITO A +7 more
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Abstract Self-luminous fluorophores allow us to stain with high contrast using multiple labels of high specificity and sensitivity. Similarly, fluorescent proteins allow sophisticated labelling of tissues, cells and sub-cellular components. Fluorescent proteins permit marking of proteins.
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2005
Fluorescence microscopy is the essential technique for investigation of the intracellular distribution of macromolecules and various organelles also in yeast cells. In this chapter, detailed practical procedures for fluorescence microscopic observations developed or adopted in our laboratory are described.
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Fluorescence microscopy is the essential technique for investigation of the intracellular distribution of macromolecules and various organelles also in yeast cells. In this chapter, detailed practical procedures for fluorescence microscopic observations developed or adopted in our laboratory are described.
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Fundamentals and developments in fluorescence-guided cancer surgery
Nature Reviews Clinical Oncology, 2021Friso Achterberg +2 more
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