Results 291 to 300 of about 3,074,988 (359)
3D printed miniature attenuated total internal reflection assisted fluorescence microscopy. [PDF]
Sci RepPark DH +5 more
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Immobilization and enhancement of a heterodimeric fluorescence biosensor in fibrous protein biomaterials. [PDF]
Protein SciBooth RM +7 more
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Rasal2 inhibits autophagic-exosomes secretion via regulating Rab27a in triple-negative breast cancer progression. [PDF]
J Transl MedWang X +6 more
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Two-photon laser scanning fluorescence microscopy.
Science, 1990Molecular excitation by the simultaneous absorption of two photons provides intrinsic three-dimensional resolution in laser scanning fluorescence microscopy.
W. Denk, J. Strickler, W. Webb
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Optics Letters, 1994
We propose a new type of scanning fluorescence microscope capable of resolving 35 nm in the far field. We overcome the diffraction resolution limit by employing stimulated emission to inhibit the fluorescence process in the outer regions of the ...
S. Hell, Jan Wichmann
semanticscholar +1 more source
We propose a new type of scanning fluorescence microscope capable of resolving 35 nm in the far field. We overcome the diffraction resolution limit by employing stimulated emission to inhibit the fluorescence process in the outer regions of the ...
S. Hell, Jan Wichmann
semanticscholar +1 more source
Fluorescence tissue microscopy
Progress in Biophysics and Molecular Biology, 2022The increasing need to investigate large biological specimens like whole tissues in 3D has driven the development of novel optical techniques capable of volumetric imaging with no compromises in terms of field-of-view and spatial resolution. On the other hand, a major challenge in the imaging of bulk biological tissues is their strong scattering which ...
Sacconi, L +3 more
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Nature Methods, 2005
Although fluorescence microscopy permeates all of cell and molecular biology, most biologists have little experience with the underlying photophysical phenomena. Understanding the principles underlying fluorescence microscopy is useful when attempting to solve imaging problems.
Jeff W, Lichtman, José-Angel, Conchello
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Although fluorescence microscopy permeates all of cell and molecular biology, most biologists have little experience with the underlying photophysical phenomena. Understanding the principles underlying fluorescence microscopy is useful when attempting to solve imaging problems.
Jeff W, Lichtman, José-Angel, Conchello
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2019
In this two-part chapter, the background to confocal microscopy and two-photon fluorescence microscopy is first presented, with a detailed description of the optical setup. This is followed by a critical account of the many super-resolution techniques: coordinated stochastic fluorescence microscopy (photoactivation localization microscopy ...
Alberto Diaspro +7 more
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In this two-part chapter, the background to confocal microscopy and two-photon fluorescence microscopy is first presented, with a detailed description of the optical setup. This is followed by a critical account of the many super-resolution techniques: coordinated stochastic fluorescence microscopy (photoactivation localization microscopy ...
Alberto Diaspro +7 more
openaire +4 more sources
Current Protocols in Immunology, 1998
AbstractAbsorption of energy as light by some molecules and emission of energy as fluorescence can occur only at certain wavelengths, which are characteristic for a given molecule (fluorophore). Fluorescence microscopes are equipped to observe the fluorescence of one or more specific fluorophores to localize specific molecules and analyzed cellular ...
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AbstractAbsorption of energy as light by some molecules and emission of energy as fluorescence can occur only at certain wavelengths, which are characteristic for a given molecule (fluorophore). Fluorescence microscopes are equipped to observe the fluorescence of one or more specific fluorophores to localize specific molecules and analyzed cellular ...
openaire +5 more sources