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2013
Optical microscopy has developed as an indispensable tool for Arabidopsis cell biology. This is due to the high sensitivity, good spatial resolution, minimal invasiveness, and availability of autofluorescent proteins, which can be specifically fused to a distinct protein of interest.
Sébastien, Peter +2 more
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Optical microscopy has developed as an indispensable tool for Arabidopsis cell biology. This is due to the high sensitivity, good spatial resolution, minimal invasiveness, and availability of autofluorescent proteins, which can be specifically fused to a distinct protein of interest.
Sébastien, Peter +2 more
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Fluorescence Lifetime Imaging Microscopy (FLIM)
A Practical Guide to Optical Microscopy, 2019Fluorescence Lifetime Imaging Microscopy (FLIM) produces an image based on the differences in the excited state decay rate from a fluorescent sample. Thus, FLIM is a fluorescence imaging technique where the contrast is based on the lifetime of individual
John Girkin
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Fluorescent Speckle Microscopy
Cold Spring Harbor Protocols, 2011INTRODUCTIONFluorescent speckle microscopy (FSM) is a live imaging and quantitative measurement technique used for analyzing motion and turnover of macromolecular assemblies in vivo and in vitro. It differs from related imaging techniques such as photobleaching and photoactivation in its use of substantially lower concentrations of fluorescently ...
Benjamin R. Houghtaling +2 more
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Fundamentals of Fluorescence and Fluorescence Microscopy
2003This chapter discusses the fundamental physics of fluorescence. The application of fluorescence to microscopy represents an important transition in the development of microscopy, particularly as it applies to biology. It enables quantitating the amounts of specific molecules within a cell, determining whether molecules are complexing on a molecular ...
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Factors in Fluorescence Microscopy
Journal of the Optical Society of America, 1947This paper is concerned with a presentation and an attempted evaluation of the various factors governing successful observations with the microscope using fluorescence technics. The primary considerations are: (1) brightness of the fluorescent image in absolute terms and (2) brightness level, image contrast and resolving power of the eye.
Charles Sheard, D. H. Hamly
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Digital Fluorescence Microscopy
Current Protocols in Cytometry, 1997AbstractFluorescence microscopy is a valuable tool for biomedical research, providing good sensitivity and high multiplicity. Specialized techniques (anisotropy measurement, resonance energy transfer) can provide information on cell spatial arrangement or label microenvironment.
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Nature Methods, 2005
Fluorescence microscopy has undergone a renaissance in the last decade. The introduction of green fluorescent protein (GFP) and two-photon microscopy has allowed systematic imaging studies of protein localization in living cells and of the structure and function of living tissues.
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Fluorescence microscopy has undergone a renaissance in the last decade. The introduction of green fluorescent protein (GFP) and two-photon microscopy has allowed systematic imaging studies of protein localization in living cells and of the structure and function of living tissues.
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Sparse deconvolution improves the resolution of live-cell super-resolution fluorescence microscopy
Nature Biotechnology, 2021Weisong Zhao +27 more
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Light sheet fluorescence microscopy
Nature Reviews Methods Primers, 2021Ernst H. K. Stelzer +6 more
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Fluorescence (Multiwave) Confocal Microscopy
Dermatologic Clinics, 2016In addition to reflectance confocal microscopy, multiwave confocal microscopes with different laser wavelengths in combination with exogenous fluorophores allow fluorescence mode confocal microscopy in vivo and ex vivo. Fluorescence mode confocal microscopy improves the contrast between the epithelium and the surrounding soft tissue and allows the ...
Welzel, Julia +2 more
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