Results 81 to 90 of about 2,197,628 (281)
High-veracity functional imaging in scanning probe microscopy via Graph-Bootstrapping
Nature Communications, 2018 Scanning probe microscopy methods can generate high-dimensional data sets that correspond to a low-dimensional sample. Here, Li et al. develop a graphical bootstrapping method to quantitatively visualize large-scale high-dimensional datasets.
Xin Li +5 more
doaj +1 more source
FEBS Open Bio, EarlyView.Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas +6 more
wiley +1 more source
Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley +1 more source
Combining scanning probe microscopy and x-ray spectroscopy
Nanoscale Research Letters, 2011 A new versatile tool, combining Shear Force Microscopy and X-Ray Spectroscopy was designed and constructed to obtain simultaneously surface topography and chemical mapping.
Bjeoumikhov Aniouar +12 more
doaj
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
SoftwareX, 2016 In the last decades scanning probe microscopy and spectroscopy have become well-established tools in nanotechnology and surface science. This opened the market for many commercial manufacturers, each with different hardware and software standards ...
Michael Ruby
doaj +1 more source
Probing single biomolecules with atomic force microscopy [PDF]
, 1997 Fritz J, Anselmetti D, Jarchow J, Fernandez-Busquets X. Probing single biomolecules with atomic force microscopy. Journal of structural biology. 1997;119(2):165-171.During the last years, atomic force microscopy (AFM) has developed from a microscopy tool
Fritz, Jürgen +3 more
core +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma
FEBS Open Bio, EarlyView.GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain +11 more
wiley +1 more source