Results 131 to 140 of about 1,587,352 (256)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Enhancing urothelial carcinoma diagnosis with artificial intelligence-integrated urine cytology: Biopsy-validated accuracy and efficiency gain. [PDF]

Cancer Cytopathol
Lajara S, Cuda J, Geisler DL, Staniszewski C, Yang WL, Atkison K, Lin CY, Shao G, Liu TJ, Crothers B, Dhir R, Khader SN.   +11 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Evaluation of microscopy and first response™ malaria Ag PAN/Pf RDT compared to qPCR and hemoglobin levels in pregnant women at delivery in Busia County, Western Kenya. [PDF]

BMC Infect Dis
Kipchirchir D, Wakoli DM, Imbusi G, Makandi R, Chimwani E, Chumbe SA, Ghebremichael M, Ong'echa JM, Bargul JL, Idris AH, Ondigo BN.   +10 more
europepmc   +1 more source

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

FEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima, Gustavo Narvaes Guimarães, Emilyane de Oliveira Santana Amaral, Bianca Caroline Figueiredo Bianco, Manuel Jara‐Espejo, Sérgio Roberto Peres Line, Ana Paula de Souza, Aline Cristiane Planello   +7 more
wiley   +1 more source

A brief history of the human liver peroxisome. [PDF]

Histochem Cell Biol
De Craemer D, Van den Branden C, Roels F.   +2 more
europepmc   +1 more source

Risk Prediction Models for Recurrence After Curative Treatment of Early‐Stage or Locally Advanced Lung Cancer: A Systematic Review

Aging and Cancer, EarlyView.
This systematic review synthesizes prognostic models for survival and recurrence in resected non‐small cell lung cancer. While many models demonstrate moderate to good discrimination, few are externally validated and reporting quality is variable, limiting clinical applicability and highlighting the need for robust, transparent model development ...
Evangeline Samuel, S. Tissera, E. Paul, J. Zalcberg, R. G. Stirling   +4 more
wiley   +1 more source

A cytosolic IF1 reporter enables real-time visualization of severe mitochondrial membrane damage. [PDF]

J Cell Biol
Gao E, Guan L, Zhang K, Qi S, Xu J, Zhang J, Zhu T, Yang B, Yang C, Miska E, Zhang M, Xu S.   +11 more
europepmc   +1 more source

A Prospective Study of Individuals at Risk of Multiple Sclerosis Informs the Design of Primary Prevention Studies

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective In multiple sclerosis, the optimal time for deploying a therapeutic intervention is before the central nervous system is damaged; given the success of trials treating the earliest stage of MS, the radiologically isolated syndrome, developing primary prevention strategies is an important next challenge.
Amy W. Laitinen, Ceren Tozlu, Tina Roostaei, Fatoumata Diallo, Kaho Onomichi, Jiyeon Son, Zongqi Xia, Philip L. De Jager   +7 more
wiley   +1 more source

Comparative Evaluation of Smear Microscopy, Xpert MTB/RIF, Löwenstein-Jensen Culture, and MGIT 960 for the Diagnosis of Pulmonary and Extrapulmonary Tuberculosis in Tangier, Morocco. [PDF]

Cureus
Amrani Souhli R, Sabri H, Lazraq S, Bahyat K, Gorfti B, Rissoul K.   +5 more
europepmc   +1 more source