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Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy.

Optics Letters, 1994
We propose a new type of scanning fluorescence microscope capable of resolving 35 nm in the far field. We overcome the diffraction resolution limit by employing stimulated emission to inhibit the fluorescence process in the outer regions of the ...
S. Hell, Jan Wichmann
semanticscholar   +1 more source

Scanning Electron Microscopy and X-Ray Microanalysis

, 1992
A clear description of the field of scanning electron microscopy and X-ray microanalysis, including coverage of specimen preparation, electron emission, lenses and electromagnetic fields, specimen-beam interactions, detectors, image construction, image ...
Robert Edward Lee
semanticscholar   +1 more source

Chemical Microscopy

Analytical Chemistry, 1986
This is a review of the publications in chemical microscopy from January 1984 through December 1985, primarily as documented in Chemical Abstracts. The review stresses advances in microscopical techniques, primarily using the polarized light microscope, and in instrumentation related to present or potential analytical methods.
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scanning tunneling microscopy

Catalysis from A to Z, 2020
The invention of the scanning tunneling microscope was a singularity event in the field of surface science and condensed matter physics. The ability to visualize individual atoms in an atomic structure was a huge step forward in experimental development,
R. Schlögl
semanticscholar   +1 more source

Methods of Digital Video Microscopy for Colloidal Studies

, 1996
We describe a set of image processing algorithms for extracting quantitative data from digitized video microscope images of colloidal suspensions. In a typical application, these direct imaging techniques can locate submicrometer spheres to within 10 nm ...
J. Crocker, D. Grier
semanticscholar   +1 more source

Transmission Electron Microscopy

, 2008
Particle Optics of Electrons.- Wave Optics of Electrons.- Elements of a Transmission Electron Microscope.- Electron-Specimen Interactions..- Scattering and Phase Contrast.- Theory of Electron Diffraction.- Electron-Diffraction Modesand Applications ...
Ludwig Reimer, Helmut Kohl
semanticscholar   +1 more source

Embedding in epoxy resins for ultrathin sectioning in electron microscopy.

Stain Technology, 1960
Fixed tissue is dehydrated with tertiary butyl alcohol overnight. The following day it is cleared in toluene, infiltrated and embedded in Araldite resin-hardener-accelerator mixture without dibutyl phthalate, and polymerized at 60° C.
K. C. Richardson, L. Jarett, E. Finke
semanticscholar   +1 more source

Microscopy

Pancreatology, 2009
Microscopy employs the use of microscopes, which are instruments designed to produce magnified visual or photographic images of objects too small to be seen with the naked eye. The goal of the microscope is to accomplish three tasks, namely produce a magnified image of the specimen, separate the details in the image, and render the details visible to ...
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Microscopy Techniques

2016
Introduzione a pag ...
Feola A   +4 more
openaire   +3 more sources

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