Results 41 to 50 of about 2,430,479 (305)

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Cunning Pathogen Tactics: Neutrophil Movement Influenced by Bacterial Attractants in the Experimental Model

Cellular Microbiology
To explore the peculiarities of neutrophil motility, two models of chemoattraction were created: a horizontal model, where a container with bacterial chemoattractant was attached laterally to the endotheliocyte monolayer, and a vertical model, simulating
Svetlana N. Pleskova, Nikolay А. Bezrukov, Ekaterina N. Gorshkova, Eseniya V. Otstavnova, Dmitry V. Novikov   +4 more
doaj   +1 more source

python-microscopy/python-microscopy: Release 23.02.20

, 2023
The python-microscopy environment - an open-source package for light ...
barentine, Mick, Akalanka, adrianhollow, Kenny Chung, actions-user, David Baddeley, Zach Marin, David Miguel Susano Pinto, csoeller, Yujin-Bao   +10 more
core   +1 more source

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

In situ observation of catalyst nanoparticle sintering resistance on oxide supports via gas phase transmission electron microscopy

Applied Microscopy
Oxide-supported metal catalysts are essential components in industrial processes for catalytic conversion. However, the performance of these catalysts is often compromised in high temperature reaction environments due to sintering effects.
Wonjun Kim, Kangsik Kim, Jaejin Kim, Zonghoon Lee   +3 more
doaj   +1 more source

Some living eukaryotes during and after scanning electron microscopy

Applied Microscopy, 2021
Electron microscopy (EM) is an essential imaging method in biological sciences. Since biological specimens are exposed to radiation and vacuum conditions during EM observations, they die due to chemical bond breakage and desiccation.
Ki Woo Kim
doaj   +1 more source

Transferrin receptor 1‐mediated iron uptake supports thermogenic activation in human cervical‐derived adipocytes

FEBS Letters, EarlyView.
In this study, we found that human cervical‐derived adipocytes maintain intracellular iron level by regulating the expression of iron transport‐related proteins during adrenergic stimulation. Melanotransferrin is predicted to interact with transferrin receptor 1 based on in silico analysis.
Rahaf Alrifai, Mizuki Seo, Gyath Karadsheh, Fachrur Rizal Mahendra, Máté Á Demény, Ferenc Győry, János András Mótyán, László Fésüs, Endre Kristóf, Rini Arianti   +9 more
wiley   +1 more source

Electron diffraction and dark-field TEM for structural analysis of 2D van der Waals materials

Applied Microscopy
Two-dimensional (2D) van der Waals materials possess structural degrees of freedom that set them apart from conventional bulk crystals and strongly influence their physical properties.
Byunghyun Kim, Daesung Park, Siwon Jeong, Hyobin Yoo   +3 more
doaj   +1 more source

Lightsheet Microscopy

Current Protocols, 2022
AbstractIn this paper, we review lightsheet (selective plane illumination) microscopy for mouse developmental biologists. There are different means of forming the illumination sheet, and we discuss these. We explain how we introduced the lightsheet microscope economically into our core facility and present our results on fixed and living samples.
Laura, Dyer, Andrew, Parker, Keanu, Paphiti, Jeremy, Sanderson   +3 more
openaire   +2 more sources

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source