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Genome-Wide Identification of the <i>WUSCHEL-Related Homeobox</i> (<i>WOX</i>) Gene Family in Barley Reveals the Potential Role of <i>HvWOX8</i> in Salt Tolerance. [PDF]
Zhang W +6 more
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Haploid Production in <i>Cannabis sativa</i>: Recent Updates, Prospects, and Perspectives. [PDF]
Ahsan SM +6 more
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Microspore Embryogenesis in Citrus
2021This chapter deals with microspore embryogenesis in Citrus. Microspore embryogenesis allows to induce immature gametes (microspores) and to deviate them, in this case, the male one, from the normal gametophytic developmental route in the direction of the sporophytic one, yielding homozygous organisms (embryos and plants).
Yahyaoui E, Germanà Maria
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Barley Isolated Microspore Culture
2021The production of doubled haploids (DHs) has proved to be a highly valuable tool to obtain new cultivars. Among the cereals, barley (Hordeum vulgare L.) is the most successful species in large-scale haploid production. Techniques employed for this purpose are based on either the gynogenetic or the androgenetic pathway.
Luís, Cistué, Begoña, Echávarri
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Genetic manipulation of microspores and microspore-derived embryos
In Vitro Cellular & Developmental Biology - Plant, 1992Recent advances in plant cell and molecular biology have furthered the genetic manipulation of many plant species and advanced the options for crop improvement. Among the many targets for genetic manipulation, microspores offer several unique advantages: they are haploid, single-celled, and highly synchronized. In many plant species microspores develop
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Plant Science, 1995
The present review will examine some of the recent developments in cereal microspore culture. Experimental procedures as well as applied purposes will be considered. This article will mainly concentrate on the culture of isolated microspores, but due to the close relationship some aspects of anther culture will be discussed also.
Alwine Jähne, Horst Lörz
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The present review will examine some of the recent developments in cereal microspore culture. Experimental procedures as well as applied purposes will be considered. This article will mainly concentrate on the culture of isolated microspores, but due to the close relationship some aspects of anther culture will be discussed also.
Alwine Jähne, Horst Lörz
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PCIB an Antiauxin Enhances Microspore Embryogenesis in Microspore Culture of Brassica juncea
Plant Cell, Tissue and Organ Culture, 2006An efficient protocol to improve microspore embryogenesis is established in an important oleiferous crop, Brassica juncea (Indian mustard). Colchicine was used for enhancing microspore embryogenesis and also to obtain doubled haploid embryos. Colchicine at high concentrations (>10 mg l−1), for 24 h, proved convenient for direct recovery of diploid ...
Agarwal, P.K. +4 more
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Journal of Tissue Culture Methods, 1989
Methodologies of microspore culture that have been successfully developed in Brassica napus, Zea mays, and Hordeum vulgare, three major, important crop species. However, the protocols can be applied to other plant species effectively, perhaps with some testing and ...
Bin Huang, Wilfred A. Keller
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Methodologies of microspore culture that have been successfully developed in Brassica napus, Zea mays, and Hordeum vulgare, three major, important crop species. However, the protocols can be applied to other plant species effectively, perhaps with some testing and ...
Bin Huang, Wilfred A. Keller
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Isolated Microspore Culture in Barley
2018Isolated microspore culture (IMC) is the most efficient way to produce large numbers of doubled-haploid (DH) barley plants in a short time. Yet, while IMC is more cost-efficient and less labor-intensive than anther culture, it is technically more complex and requires more experienced personnel if it is to yield its full potential.
Esteves, Patricio, Belzile, François
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