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Microspores. Evolution and Ontogeny
Kew Bulletin, 1992Microsporogenesis - the male programme of development, S.Blackmore and R.B.Knox the phylogenetic context of microsporogenesis, P.R.Crane meiospore formation in charophycean algae, L.E.Graham sporogenesis in bryophytes, R.C.Brown and B.E.Lemmon pteridophyte sporogenesis - a survey of spore wall ontogeny and fine structure in a polyphyletic plant group ...
Simon J. Owens, S. Blackmore, R. B. Knox
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Isolated wheat microspore culture
Plant Cell, Tissue and Organ Culture, 1995The use of doubled haploid plants in a wheat breeding program requires an efficient haploid production system. While the techniques for producing doubled haploids from anther culture are well established, those for isolated microspores are complicated and inefficient.
Vicki D. Gustafson +4 more
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Brassica Improvement Through Microspore Culture
2004The microspore culture system developed for Brassica species represents one of the most efficient systems available for generating microspore-derived embryos and double haploid plants. Haploid embryos from many Brassica species can be produced at frequencies suitable for both basic research and practical applications.
Ferrie, Alison, Keller, Wilfred
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Isolated Microspore Culture in Brassica napus
2020Isolated microspore culture is the most efficient technique among those used to induce microspore embryogenesis. In the particular case of Brassica napus, it is also the most widely used and optimized. In this chapter, we describe a protocol for microspore culture in B.
Patricia, Corral-Martínez +2 more
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Nature, 1939
THE occurrence of microspores in centric diatoms and their possible nature have been the objects of great discussion. Recently, Gross1, on the basis of cultures of Chaetoceros pseudocrinitus and Ch. didymus, concluded that “there is no evidence whatever that these microspore-like bodies found in the cultures of Ch.
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THE occurrence of microspores in centric diatoms and their possible nature have been the objects of great discussion. Recently, Gross1, on the basis of cultures of Chaetoceros pseudocrinitus and Ch. didymus, concluded that “there is no evidence whatever that these microspore-like bodies found in the cultures of Ch.
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Microspore Culture in Brassica
2003Pioneering research in Brassica microspore culture (1,2,3)rapidly led to the realization that microspores provide a powerful alternative to protoplast culture as a single-celled culture method in plants. These two single-celled systems are fundamentally different, both in tissue origin and in genetic variability.
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Single-Stage Carbon Replicas of Microspores
Stain Technology, 1966Single-stage surface replicas of treated or fresh pollen grains can be made ready for the electron microscope in 1.5 hr. The microspores are discharged into a drop of 50% acetone on a 1 cm square of cleaved mica and air dried. Carbon is evaporated to a film thickness of 35 mμ during rotation of the mica support.
J R, Rowley, J J, Flynn
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Microspore development in cultured maize anthers
Plant Cell Reports, 1988The present study follows in vivo and in vitro microspore development utilizing an anther culture-responsive maize genotype (Pa91×FR16) and a DNA-specific fluorescent dye (mithramycin). Cultured anthers were sampled at various times and scored for abnormal microspore divisions, multicellular masses, and embryo-like structures. The frequency of abnormal
S M, Pescitelli, J F, Petolino
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1994
The maize pollen grain represents the beginning of a short-lived gametophytic phase during which the two sperm are delivered to the embryo sac prior to fertilization. Although this stage of the life cycle normally consists of only a few cell divisions, under certain experimental conditions, gametophytes can be induced to undergo an altered development,
J. F. Petolino, A. D. Genovesi
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The maize pollen grain represents the beginning of a short-lived gametophytic phase during which the two sperm are delivered to the embryo sac prior to fertilization. Although this stage of the life cycle normally consists of only a few cell divisions, under certain experimental conditions, gametophytes can be induced to undergo an altered development,
J. F. Petolino, A. D. Genovesi
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Heterozygous microspore-derived plants in rye
Theoretical and Applied Genetics, 1976In a particular experimental series involving anthers from F(1) hybrid plants of Secale cereale L., it was possible to induce the formation of 68 microspore-derived plantlets of which 61 were albinos and 7 green. 6 of the albino plants were haploid, whereas most of the others were diploid. All green rye plants were directly diploid and, after extensive
G, Wenzel, F, Hoffmann, E, Thomas
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