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Microspore culture of rye

2003
Rye (Secale cereale L.) is an important cereal in Europe, with approximately 90% of global rye production concentrated in this area. In subtropical low latitude areas, rye is grown primarily as a late-fall, early-spring forage and, secondarily, for grain.
S. Pulli, Y.-D. Guo
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Microspore Culture in Brassica

2003
Pioneering research in Brassica microspore culture (1,2,3)rapidly led to the realization that microspores provide a powerful alternative to protoplast culture as a single-celled culture method in plants. These two single-celled systems are fundamentally different, both in tissue origin and in genetic variability.
openaire   +2 more sources

Brassica Improvement Through Microspore Culture

2004
The microspore culture system developed for Brassica species represents one of the most efficient systems available for generating microspore-derived embryos and double haploid plants. Haploid embryos from many Brassica species can be produced at frequencies suitable for both basic research and practical applications.
Ferrie, Alison, Keller, Wilfred
openaire   +2 more sources

Cytological and ultrastructural changes induced in anther and isolated-microspore cultures in barley: Fe deposits in isolated-microspore cultures

Journal of Structural Biology, 2005
To gain further insight into the role played by sporophytic anther tissues in the early stages of the androgenic process, we have compared the cytology and ultrastructure of barley embryogenic pollen grains obtained by anther culture with those obtained by isolated-microspore culture.
A, Pulido   +5 more
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Anther and microspore culture in tobacco

2003
Serving as the oldest model species, several variants of tobacco anther and microspore culture have been developed over the years, depending on the timing of the inductive stress treatment and microspore isolation (Heberle-Bors et al., 1996). A convenient, one-step procedure of anther culture in Nicotiana tabacum consists of a cold-treatment of excised
A. Touraev, E. Heberle-Bors
openaire   +1 more source

Asparagus microspore and anther culture

2003
Asparagus is a dioecious, perennial crop where male plants generally yield more than female plants. Consequently, an all-male hybrid can have improved productivity compared to one that is dioecious. Genotypes of males and females are Mm and mm, respectively, and all-male hybrids result from crossing females and supermales (MM).
D. J. Wolyn, B. Nichols
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Anther culture and isolated microspore culture in timothy

2003
Timothy (Phleum pratense L.) is one of the basic forage grasses in the world and the most important grass in winter snow covered northern latitudes. It is considered to be allohexaploid in nature (2n=6x=42). Timothy is valued for its winter hardiness, good palatability, and moderate feeding quality.
S. Pulli, Y.-D. Guo
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Culture of Isolated Microspores of Saccharum spontaneum)

Zeitschrift für Pflanzenphysiologie, 1984
Summary Microspore culture of Saccharum spontaneum L. was investigated as a means to produce haploid plants. Isolated and cultured microspores initiated sporophytic development which in 4 to 6 weeks resulted in procalli and calli. The percentage of microspores producing multicellular procalli was increased by removing the nonviable microspores ...
Maud A.W. Hinchee, Maureen M.M. Fitch
openaire   +1 more source

Isolated Microspore Culture of Maize

1994
The process of androgenesis is unique, in that haploid embryos arise from single gametophytic cells. Clear evidence for the unicellular origin of somatic embryos is lacking for most regeneration systems (Fransz and Schel 1991); therefore, totipotent microspores offer a potentially useful subject for both basic and applied studies.
S. M. Pescitelli   +2 more
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Triticale Isolated Microspore Culture for Doubled Haploid Production

2021
Here, we describe a method of triticale isolated microspore culture for production of doubled haploid plants via androgenesis. We use this method routinely because it is highly efficient and works well on different triticale genotypes. To force microspores into becoming embryogenic, we apply a 21-day cold pretreatment.
Priti, Maheshwari, John D, Laurie
openaire   +2 more sources

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