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Isolated Microspore Culture in Brassica napus
2020Isolated microspore culture is the most efficient technique among those used to induce microspore embryogenesis. In the particular case of Brassica napus, it is also the most widely used and optimized. In this chapter, we describe a protocol for microspore culture in B.
Patricia, Corral-Martínez +2 more
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Microspore development in cultured maize anthers
Plant Cell Reports, 1988The present study follows in vivo and in vitro microspore development utilizing an anther culture-responsive maize genotype (Pa91×FR16) and a DNA-specific fluorescent dye (mithramycin). Cultured anthers were sampled at various times and scored for abnormal microspore divisions, multicellular masses, and embryo-like structures. The frequency of abnormal
S M, Pescitelli, J F, Petolino
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Isolated Microspore Culture in Barley
2018Isolated microspore culture (IMC) is the most efficient way to produce large numbers of doubled-haploid (DH) barley plants in a short time. Yet, while IMC is more cost-efficient and less labor-intensive than anther culture, it is technically more complex and requires more experienced personnel if it is to yield its full potential.
Esteves, Patricio, Belzile, François
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PCIB an Antiauxin Enhances Microspore Embryogenesis in Microspore Culture of Brassica juncea
Plant Cell, Tissue and Organ Culture, 2006An efficient protocol to improve microspore embryogenesis is established in an important oleiferous crop, Brassica juncea (Indian mustard). Colchicine was used for enhancing microspore embryogenesis and also to obtain doubled haploid embryos. Colchicine at high concentrations (>10 mg l−1), for 24 h, proved convenient for direct recovery of diploid ...
Agarwal, P.K. +4 more
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Brassica Improvement Through Microspore Culture
2004The microspore culture system developed for Brassica species represents one of the most efficient systems available for generating microspore-derived embryos and double haploid plants. Haploid embryos from many Brassica species can be produced at frequencies suitable for both basic research and practical applications.
Ferrie, Alison, Keller, Wilfred
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MICROSPORE CULTURE IN ELEVEN CULTIVARS OF LOQUAT
Acta Horticulturae, 2015In plant breeding, microspore embryogenesis via isolated microspore culture is increasing as a method to obtain in a single-step doubled haploids and homozygosity. In this study, isolated microspore culture of eleven cultivars of loquat (Eriobotrya japonica (Thunb.) Lindl.) has been carried out.
Blasco, M. +7 more
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1994
The maize pollen grain represents the beginning of a short-lived gametophytic phase during which the two sperm are delivered to the embryo sac prior to fertilization. Although this stage of the life cycle normally consists of only a few cell divisions, under certain experimental conditions, gametophytes can be induced to undergo an altered development,
J. F. Petolino, A. D. Genovesi
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The maize pollen grain represents the beginning of a short-lived gametophytic phase during which the two sperm are delivered to the embryo sac prior to fertilization. Although this stage of the life cycle normally consists of only a few cell divisions, under certain experimental conditions, gametophytes can be induced to undergo an altered development,
J. F. Petolino, A. D. Genovesi
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Nuclear fusion in cultured microspores of barley
Plant Cell Reports, 1987Fusion of the generative and vegetative nuclei physically separated by a wall has been observed in cultured microspores of barley. The generative cell appears to play an active role in fusion as it elongates toward the vegetative nucleus, becomes detached from the microspore wall, and finally completely encloses the vegetative nucleus.
Lee, Feng-Ming, Chen, Chi-Chang
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Albinism in Microspore Culture
2008Frequent formation of albino plants from in vitro cultured microspores is a particular problem for chromosome doubled haploid production in cereals and grasses. The phenomenon was first thought to be associated with maternal inheritance of plastids visualized by large deletions and rearrangements of plastid genomes in albino plants.
A. M. Torp, S. B. Andersen
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2003
Rye (Secale cereale L.) is an important cereal in Europe, with approximately 90% of global rye production concentrated in this area. In subtropical low latitude areas, rye is grown primarily as a late-fall, early-spring forage and, secondarily, for grain.
S. Pulli, Y.-D. Guo
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Rye (Secale cereale L.) is an important cereal in Europe, with approximately 90% of global rye production concentrated in this area. In subtropical low latitude areas, rye is grown primarily as a late-fall, early-spring forage and, secondarily, for grain.
S. Pulli, Y.-D. Guo
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