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Protocol for broccoli microspore culture

2003
Broccoli (Brassica oleracea var. italica) is a vegetable cole crop of considerable interest to breeders and seed companies since its area of production has increased in recent years mainly due to improved nutritional qualities, e.g. anticancer properties. Breeding companies strive more and more to bring F1 hybrid seeds onto the market.
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Haploid wheat isolated microspore culture protocol

2003
Haploid production in hexaploid wheats (Triticum aestivum L.) has been achieved mainly by wide hybridization. The first wide hybridization system using Hordeum bulbusom pollen was very genotype specific. However, the subsequent use of maize, sorghum or millet pollinators has largely overcome this genotype effect and the system is being used in many ...
K. J. Kasha   +4 more
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Towards isolated microspores culture of red beet

Kartofel` i ovoshi, 2022
Производство удвоенных гаплоидов (УГ) in vitro – ускоренный способ создания чистых линий для селекции коммерческих F1гибридов. Наиболее распространенный метод производства удвоенных гаплоидов у растений рода Beta, главным образом свеклы сахарной, – культура изолированных семязачатков (гиногенез).
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Microspore culture of Brassica species

2003
The production of haploid plants from isolated microspores of Brassica napus (L.) was first reported by Lichter in 1982 (Lichter, 1982). The embryo yields from these initial experiments were low but have since increased through manipulation of the culture technique.
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Microspore Culture and Doubled Haploid Technology

2013
Haploids and doubled haploids (DHs) produced by in vitro culture of gametophytic cells, especially male gametophyto, are of great importance to plant breeding and basic science. The routine protocol of isolated microspore culture of Brassica has been established, and used worldwide.
Yoshihito Takahata   +2 more
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Anther and microspore culture in tobacco

2003
Serving as the oldest model species, several variants of tobacco anther and microspore culture have been developed over the years, depending on the timing of the inductive stress treatment and microspore isolation (Heberle-Bors et al., 1996). A convenient, one-step procedure of anther culture in Nicotiana tabacum consists of a cold-treatment of excised
A. Touraev, E. Heberle-Bors
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Microspore culture protocol for Indonesian Brassica oleracea

Plant Cell, Tissue and Organ Culture (PCTOC), 2011
A microspore culture protocol for Brassica oleracea of Indonesian origin (cv. ‘Kemeh’) has been successfully established. A high number of embryos formed with high microspore density i.e. 15 × 104 cells/ml. Embryo formation was improved by using flower buds (4.5–4.6 mm in length) as explants, a temperature treatment at 30.5°C for 48 h and then transfer
Budi Winarto, Jaime A. Teixeira da Silva
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Microspore culture in rapeseed (Brassica napus L.)

2003
In the 1980s, it was demonstrated that high embryo yields could be obtained by culturing isolated microspores from rapeseed Brassica napus in simple, hormone free media, without going through an intermediate callus phase (Keller et al., 1987; Pechan and Keller, 1988).
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Anther and microspore culture in Camellia japonica

1997
Haploid plants have the gametophytic chromosome number. They are of great importance for the production of homozygous plants and for mutation studies. The use of anther and microspore cultures for the induction of pollen embryogenesis hastens the production of haploids and gametoclonal variants.
M. Cristina Pedroso, M. Salomé Pais
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Embryogenesis in microspore culture of Vitis subspecies

2015
VITIS - Journal of Grapevine Research, Vol. 36 No.
Sefc, Kristina M.   +2 more
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