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Microspore embryogenesis inCitrusand other fruit crops
Acta Horticulturae, 2017 Conventional methods, involving several generations of selfing, are not applicable to produce homozygous lines in Citrus as well as in the other fruit crops, due to the high heterozygosity of the genomes, the long duration of the generation cycle, the large size, and, often, the self-incompatibility. For this reason, there is no different way to obtain
M.A. Germanà, Germana' M.
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Physiologia Plantarum, 2008
Microspore embryogenesis is the most powerful androgenic pathway to produce haploid and doubled haploid plants. To deviate a microspore toward embryogenesis, a number of factors, different for each species, must concur at the same time and place. Once induced, the microspore undergoes numerous changes at different levels, from overall morphology to ...
José M, Seguí-Simarro, Fernando, Nuez
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Microspore embryogenesis is the most powerful androgenic pathway to produce haploid and doubled haploid plants. To deviate a microspore toward embryogenesis, a number of factors, different for each species, must concur at the same time and place. Once induced, the microspore undergoes numerous changes at different levels, from overall morphology to ...
José M, Seguí-Simarro, Fernando, Nuez
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Chromosoma, 2004 8 páginas, 5 figuras -- PAGS nros. 342-349A multidisciplinary study was carried out to analyse the chromosome doubling process during the early stages of in vitro maize microspore embryogenesis.
Maria C Risueño, E Matthys‐Rochon
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Initiation of microspore embryogenesis by stress
Trends in Plant Science, 1997Microspores have the remarkable capacity to develop into haploid plants via embryogenesis in vitro . Stress treatment acts as a trigger for inducing this sporophytic pathway, preventing the development of fertile pollen (gametophytic pathway). The doubled haploids generated are completely homozygous, and represent an important tool for research in ...
Alisher Touraev +2 more
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Journal of Experimental Botany, 2019 39 p.-5 fig.Under stress, isolated microspores are reprogrammed in vitro towards embryogenesis, producing doubled haploid plants that are useful biotechnological tools in plant breeding as a source of new genetic variability, fixed in homozygous plants ...
Pilar S Testillano
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Embryogenesis in callus derived from rice microspores
Plant Cell Reports, 1982Differentiating calli derived from rice (Oryza sativa L.) microspores were examined histologically. Shoot and root meristems were observed to be arising by both organogenesis as well as embryogenesis. Embryoid attachment to callus (as well as other embryoids) was at the scutellum adjacent to the mesocotyl and radicle.
A D, Genovesi, C W, Magill
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Proteomics in Rapeseed Microspore Embryogenesis
2008This chapter reviews the proteomics research performed to elucidate the molecular processes associated with embryo initiation and development in Brassica napus (rapeseed) microspore-derived embryo cultures. Classical biochemical methods such as combined [35S]-methionine labelling and 2-D gel electrophoresis (2-DE) have been used for the large-scale ...
J. Cordewener +4 more
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Colchicine-induced microspore embryogenesis in coffee
Plant Cell, Tissue and Organ Culture, 2002A protocol for the induction of androgenesis and plant regeneration from C. arabica cv. Caturra isolated microspores in vitro using colchicine pretreatment has been developed. Microspores were mechanically isolated and then carefully purified. Before colchicine pretreatment, microspores were cultured in a semi-solid medium for further develop and ...
J.C. Herrera +4 more
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Induction of embryogenesis in isolated microspores of tulip
Plant Science, 1994Abstract Isolated microspores of tulip ( Tulipa gesneriana ) cultivars ‘Leen van der Mark’ and ‘Rosario’ could be induced to form embryos. Microspores of a young developmental stage, i.e. early and mid unicellular, were the most responsive. The first sporophytic divisions, resulting in microspores with three or four vegetative-like nuclei were ...
van den Bulk, R.W. +3 more
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