5-azacytidine promotes microspore embryogenesis initiation by decreasing global DNA methylation, but prevents subsequent embryo development in rapeseed and barley [PDF]
Microspores are reprogrammed by stress in vitro towards embryogenesis. This process is an important tool in breeding to obtain double-haploid plants. DNA methylation is a major epigenetic modification that changes in differentiation and proliferation. We
María-Teresa eSolís +4 more
doaj +3 more sources
Current insights into hormonal regulation of microspore embryogenesis [PDF]
Plant growth regulator (PGR) crosstalk and interaction with the plant’s genotype and environmental factors play a crucial role in microspore embryogenesis (ME), controlling microspore-derived embryo differentiation and development as well as haploid ...
Iwona eŻur +3 more
doaj +3 more sources
Microspore embryogenesis in vitro: the role of stresses
Gametic embryogenesis is one form of totipotency of plant cells, in which either male or female gametes are induced to form embryoids (sporophytes). Regeneration of haploid plants from embryoids and subsequent chromosome duplication result in doubled ...
T. I. Djatchouk +4 more
doaj +3 more sources
Effect of temperature shock on soybean microspore embryogenesis [PDF]
In this study the effect of cold and heat shock on androgenetic induction in soybean anther culture was tested. Anthers of soybean were subjected to 4 and 33ºC, while the anthers used for control were maintained at 25ºC.
Ana Paula de Moraes +3 more
doaj +5 more sources
EMBRYOGENESIS IN CULTURE OF ISOLATED MICROSPORE OF BROCCOLI
The process of embryogenesis and technological experimental protocol has been studied and applied to produce doubled haploid plants from microspore cultured in vitro in broccoli B. oleracea L. convar. botrytis (L.) Alef. var. italica Plenck. It was shown
E. A. Domblides +5 more
doaj +3 more sources
Impact of Ionic Liquids on Induction of Wheat Microspore Embryogenesis and Plant Regeneration
Ionic liquids are novel compounds with unique chemical and physical properties. They can be received based on synthetic auxins like 2,4-dichlorophenoxyacetic acid or dicamba, which are commonly used hormones in microspore embryogenesis.
Dorota Weigt +4 more
doaj +1 more source
Microspore embryogenesis in Apiaceae
Haploid technology is used to develop uniform, true-breeding lines, as well as to accelerate crop improvement programs. Among 20 Apiaceae species screened for response to doubled haploidy, 11 generated microspore-derived embryos, and all but one of the latter yielded doubled haploid plants.
A. M. R. Ferrie +2 more
openaire +3 more sources
Microspore embryogenesis is a process of cell reprogramming, totipotency acquisition and embryogenesis initiation, induced in vitro by stress treatments and widely used in plant breeding for rapid production of doubled-haploids, but its regulating ...
Eduardo Berenguer +5 more
doaj +1 more source
Transcriptomics integrated with metabolomics reveals the effect of cold stress on rice microspores
Background Microspore culture is one of the important biotechnological tools in plant breeding. The induction of microspore embryogenesis is a critical factor that affects the yield of microspore-derived embryo productions.
Yingbo Li +7 more
doaj +1 more source
Proteomic variations after short-term heat shock treatment reveal differentially expressed proteins involved in early microspore embryogenesis in cabbage (Brassica oleracea) [PDF]
Microspore embryogenesis (ME), a widely used haploid breeding method that can considerably shorten the breeding cycle, provides an efficient mean of cultivating many important Brassica crops, such as cabbage, Chinese cabbage, and oilseed rape.
Henan Su +6 more
doaj +2 more sources

