Gametic and somatic embryogenesis through in vitro anther culture of different Citrus genotypes [PDF]
: In vitro tissue culture represents a useful technique for advancing Citrus breeding and propagation. Among in vitro regeneration systems, anther culture is commonly used to produce haploids and doubled haploids for a fast-track producing homozygous ...
Abdelgalel, A.M. +6 more
core +1 more source
The Roads to Haploid Embryogenesis
Although zygotic embryogenesis is usually studied in the field of seed biology, great attention has been paid to the methods used to generate haploid embryos due to their applications in crop breeding.
Kun Shen, Mengxue Qu, Peng Zhao
doaj +1 more source
Morphogenic microspore as an initial cell of androgenesis in vitro: review of the problem [PDF]
The phenomenon of androgenesis in vitro is widely used in biotechnological investigations of commercially valuable plants. When solving the specific tasks it is important to enter into the culture in vitro anthers containing sporogenous cells which are ...
Kruglova, N. N. +2 more
core +2 more sources
Production of embryos from microspore cultures of Portuguese tronchuda cabbage landraces [PDF]
Twelve accessions of tronchuda cabbage landraces were tested for their ability to produce embryos through microspore culture in NLN-13 medium. A sample from all the isolations was stained with DAPI for determination of microspore developmental stage. The
Dias, J. S., Vicente, Joana G.
core +1 more source
Ovary pre-conditioned medium and ovary co-culture increased the efficiency of green doubled haploid plant production in bread wheat anther culture. The positive effect of this medium led to a 6- and 11-fold increase in the numbers of embryos and green ...
Ana María Castillo +2 more
doaj +1 more source
Cytological development, calcium dynamic and metabolite profile of stress-induced tobacco (Nicotiana tabacum L.) microspore under calcium chloride treatment [PDF]
Exogenous calcium is pivotal in activating microspore embryogenesis via calcium signaling. Altering the external calcium levels in the medium has been documented to impact the success rate of microspore embryogenesis initiation.
C. T. Rahmawati +6 more
doaj +1 more source
CENH3-GFP: a visual marker for gametophytic and somatic ploidy determination in Arabidopsis thaliana [PDF]
Background: The in vivo determination of the cell-specific chromosome number provides a valuable tool in several aspects of plant research. However, current techniques to determine the endosystemic ploidy level do not allow non-destructive, cell-specific
Angenon, Geert +4 more
core +5 more sources
The low molecular weight fraction of compounds released from immature wheat pistils supports barley pollen embryogenesis [PDF]
Pollen embryogenesis provides a useful means of generating haploid plants for plant breeding and basic research. Although it is well established that the efficacy of the process can be enhanced by the provision of immature pistils as a nurse tissue, the ...
Hans-Peter Mock +3 more
core +2 more sources
Teknik Kultur Anther pada Pemuliaan Anthurium [PDF]
. Kultur anther merupakan teknik baru yang telah dikembangkan pada beberapa tanaman untuk mendapatkan galur murni melalui produksi tanaman haploid ganda.
Rachmawati, F. (Fitri) +1 more
core +2 more sources
Microspore embryogenesis in barley: anther pre-treatment stimulates plant defence gene expression [PDF]
Microspore embryogenesis (ME) is a process in which the gametophytic pollen programme of the microspore is reorientated towards a new embryo sporophytic programme.
Boutilier, K.A. +3 more
core +2 more sources

