Mastering the art of sectioning: a comprehensive guide to slide-microtome technology and histological applications [PDF]
Frontiers in Veterinary ScienceThe well-known technique of microtomy, which is an essential cutting tool, was first developed for light and transmission electron microscope uses, but it is currently also utilized to prepare specimens for atomic force microscopy (AFM), ion microscopy ...
Yasmin M. Abd El-Aziz +9 more
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The rapid-tome, a 3D-printed microtome, and an updated hand-sectioning method for high-quality plant sectioning [PDF]
Plant Methods, 2023 Background Microscopic analysis of plant anatomy is a common procedure in biology to study structure and function that requires high-quality sections for accurate measurements.
David J. Thomas +2 more
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Harvesting mouse suprachiasmatic nucleus by vibrating microtome for diurnal transcriptome analysis
STAR Protocols, 2023 Summary: The mammalian suprachiasmatic nucleus (SCN) is the principal circadian clock that synchronizes daily behavioral and physiological responses in response to environmental cues.
Akanksha Bafna +3 more
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Combination of focused ion beam (FIB) and microtome by ultrathin slice preparation for transmission electron microscopy (TEM) observation [PDF]
Earth, Planets and Space, 2018 There are growing demands for integrated study of isotopes, trace elements and crystallography of micron-sized grains observed on polished sections or scattered on surface of sample holders.
Yuchen Xu +4 more
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Protocol for the establishment and morphological characterization of long-term cultivated murine cerebral organoids [PDF]
STAR ProtocolsSummary: Murine cerebral organoids provide a rapid and reproducible in vitro system that recapitulates key aspects of neurogenesis. While human cerebral organoid protocols are well established, methods for non-human models remain limited.
Issam El-Debs +4 more
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A centrifugal ice microtome for measurements of atmospheric CO2 on air trapped in polar ice cores [PDF]
Atmospheric Measurement Techniques, 2013 For atmospheric CO2 reconstructions using ice cores, the technique to release the trapped air from the ice samples is essential for the precision and accuracy of the measurements.
H. Fischer, T. F. Stocker, B. Bereiter
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A Protocol for Laser-Assisted Microdissection and tRF & tiRNA Sequencing in Lung Adenocarcinoma [PDF]
Bio-ProtocolLaser-assisted microdissection (LAM) coupled with next-generation sequencing technologies offers a powerful approach to dissecting the complex cellular heterogeneity within lung adenocarcinoma (LUAD) tumors.
Zi Wang +4 more
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CELS-3D-Cutting edge light source for exciting fluorescence in microtome-based 3D microscopy and targeted correlative microscopy. [PDF]
J AnatDenyshchenko V +7 more
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Horticulturae, 2022 This first-attempt study used microtome-based methods to generate a thin cell layer culture for the micropropagation of Phal. Hwafeng Redjewel × Phal. New Cinderella.
Kuo-Chin Lo +3 more
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Targeted 2D histology and ultrastructural bone analysis based on 3D microCT anatomical locations
MethodsX, 2021 Histological processing of mineralised tissue (e.g. bone) allows examining the anatomy of cells and tissues as well as the material properties of the tissue. However, resin-embedding offers limited control over the specimen position for cutting. Moreover,
I. Moreno-Jiménez +4 more
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